Ξ²-glucosidase (gene bgl2) from Thermoascus aurantiacus IFO9748
Enzyme Description
Extremophile
Yes
thermophilic organism
EC Number
Sequence
MACAIALTAVSLFLSSVFSSPIDTSGNVRRDSVPTGYESPPYYPAPPGGWVSDWAPAYAKAYEVVSNMTLAEKVNMTTGTGIFMGPCVGQTGSALRFGIPNLCLMDSPLGIRNTDHNTAFPAGITVGATFDKELMYARGVALGEEARGKGVNVLMGPMVGPIGRKPRGGRNWEGFGADPTLQAIGGAQTIKGMQSTGVIATLKHFIGNEQEMYRMTSVIQRGYSSNIDDRTLHELYLWPFAEGVRAGVGSVMMAYNDVNGSACSQNSKLINGILKDELGFQGFVMTDWLTQIGGVSSALAGLDMAMPGDGPIPLFGDSYWGSELSRAVLNGTVPVDRLNDMVTRIVATWYKFGQDKDFPLPNFSSNTDAATGLLYPGAVFSPIGVVNQFVDVQGDHKVVARAIARDAITLLKNEDNALPLKRNDSLKIFGTDAGTNPDGINSCADKGCDKGVLTMGWGSGTSKLPYLNTPQEAIANASSNAEFFVTDSFPSNVNANPEDIAIVFINADSGENYITVEGNYGDRSAAGLYAWHNGDDLVKAAAAKFSKVVVVVHTVGPIILENWIDLPSVKSVVFAHLPGQEAGDSLVDVLFGDYSPSGHLPYTIPRSEDQYPSSVSLINQPFGQIQDTFTEGLYIDYRHFLHANLTPRYPFGHGLSYTTFNITEATISVVIPLQSEYPPTRPPKGPTPTYNNVIPPASEVAWPENFDRIWRYIYPYLDNPAAATSTAPYPYPTGYTTVPQPAPRAGGAEGGNPALWDVAFSVRVKVSNTGSRPGRAVAQLYVELPSSLGLDTPRLQLRQFEKTKTLAPGESEVLTMELTRKDLSVWDVVVQDWKAPLNGQGVKVWIGESVADLRVVCEVGRECRNI
Jiong Hong et al. (2006)
β Unusual hydrophobic linker region of Ξ²-glucosidase (BGLII) from Thermoascus aurantiacus is required for hyper-activation by organic solvents
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-006-0428-0 β
Β· Activity - Classical
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Database ID
UniProt: Q4U4W7 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris KM71H
Experimental Data (20 measurements)
20 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 2-Methyl-1-Pentanol | 20% | 1.0 | 13.2 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Diethyl Ether | 20% | 1.0 | 11.6 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | n-Hexane | 20% | 1.0 | 4.65 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 1.0 | 1.6 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Cyclohexane | 20% | 1.0 | 3.64 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Acetone | 20% | 1.0 | 7.29 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Allyl Alcohol | 20% | 1.0 | 6.79 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Octanol | 20% | 1.0 | 54.7 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Heptanol | 20% | 1.0 | 48.9 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 2-Methyl-2-Pentanol | 20% | 1.0 | 8.15 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Methanol | 20% | 1.0 | 6.04 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 3-Methyl-1-Pentanol | 20% | 1.0 | 40.3 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 4-Methyl-2-Pentanol | 20% | 1.0 | 23.6 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Hexanol | 20% | 1.0 | 42.4 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Pentanol | 20% | 1.0 | 23.4 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 2-Butanol | 20% | 1.0 | 3.1 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Butanol | 20% | 1.0 | 14.1 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Isopropanol | 20% | 1.0 | 9.05 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Propanol | 20% | 1.0 | 9.29 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 20% | 1.0 | 9.7 | relative activity | 50 mM sodium acetate buffer (bit of doubt) | 5 | 40Β°C | 5 mM p-nitrophenyl Ξ²-glucose | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in relative activity) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.