Computer-aided design for enhancing the tolerance of ω-amine transaminase against methanol as a cosolvent

Shuai Qiua; Tong Wang; Cong-Lin Ju; Zhuo-Yuan Cheng; Fang-Fang Fan; Le-He Mei; Jun Huang (2025)

Bioorganic Chemistry

128 measurements across 1 enzyme  ·  doi:10.1016/j.bioorg.2025.108913

Characterization of a novel alginate lyase Alg0392 with organic solvent-tolerance from Alteromonas sp. A1-6

Tong Liang; Jing Chen; Jing Li; Ming-li Dong; Zhenggang Han; Feng-juan Shan; Xue-wang Gao; Da-zhong Yan (2025)

Applied Microbiology and Biotechnology

8 measurements across 1 enzyme  ·  doi:10.1007/s00253-025-13506-1

Methanol response of V171S lipase from Geobacillus sp. 42 explored via experimental and in silico approaches

Ummie Umaiera Mohd Johan; Siti Nor Hasmah Ishak; Wahhida Latip; Raja Noor Zaliha Raja Abd. Rahman; Abu Bakar Salleh; Adam Thean Chor Leow; Mohd Shukuri Mohamad Ali (2025)

AMB Express

26 measurements across 1 enzyme  ·  doi:10.1186/s13568-025-01966-7

Characterization of Cold-Adapted Lipase from Exiguobacterium sp. and Its Cold Adaptation Mechanism

Yanpo Li; Fang Huang; Yongguo Jin; Danjuan Wang; Yan Yao; Ok-Hwan Lee; Nikolai Kuhnert; Xing Fu (2025)

Journal of Agricultural and Food Chemistry

10 measurements across 1 enzyme  ·  doi:10.1021/acs.jafc.4c11222

Degradation and ring-opening polymerization of poly(ε-caprolactone) by a novel enzyme from Pseudomonas sp. DS0801

Yao Di; Wenfei Luan; Lihua Sun; Jing Qi; Hongmei Xia; Fan Li; Niu Zhai (2025)

iScience

6 measurements across 1 enzyme  ·  doi:10.1016/j.isci.2025.114173

Heterologous expression and enzymatic properties of lipase from Mucor circinelloides

Yao Zhang; Yan Sun; Zhuo Liu; Jiajun Leng; Qing Liu; Yuanda Song (2025)

Scientific Reports

14 measurements across 1 enzyme  ·  doi:10.1038/s41598-025-93938-x

Molecular cloning, characterization, and structural stability analysis of a rare acidic catechol 2,3-dioxygenase from the metagenome of coal-polluted soil

Yi-Xiang Wang; Bing-Xue Dong; Yi-Jia Liu; Yi-Quan Tan; Yu-Ting An; Li-Hui Lin; Gang Li (2025)

International Journal of Biological Macromolecules

15 measurements across 1 enzyme  ·  doi:10.1016/j.ijbiomac.2025.143652

Discovery and characterization of high-activity formate oxidase with low Km value under nonacidic conditions

Zhenyu Zhai; Yi-Heng P.Job Zhang (2025)

Bioscience, Biotechnology, and Biochemistry

36 measurements across 1 enzyme  ·  doi:10.1093/bbb/zbaf092

Redox and solvent-stable alkaline serine protease from Bacillus patagoniensis DB-5: heterologous expression, properties, and biotechnological applications

Zhimin Li; Yanmin Xing; Pulin Liu; Weifang Liao; Lihong Miao (2025)

Frontiers in Microbiology

7 measurements across 1 enzyme  ·  doi:10.3389/fmicb.2025.1558419

Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization

Ammar Khazaal Kadhim Almansoori; Nidyaletchmy Subba Reddy; Mustafa Abdulfattah; Sarah Solehah Ismail; Rashidah Abdul Rahim (2024)

PLOS ONE

20 measurements across 1 enzyme  ·  doi:10.1371/journal.pone.0314556

Cloning, Expression, and Characterization of a Metalloprotease from Thermophilic Bacterium Streptomyces thermovulgaris

Amna Mushtaq; Sibtain Ahmed; Tahir Mehmood; Jorge Cruz-Reyes; Amer Jamil; Shafaq Nawaz (2024)

Biology

7 measurements across 1 enzyme  ·  doi:10.3390/biology13080619

Effect of Organic Solvents on the Activity, Stability and Secondary Structure of asclepain cI, Using FTIR and Molecular Dynamics Simulations

Anabella L. Origone; Esteban G. Vega Hissi; Constanza S. Liggieri; Gerardo E. Camí; Andrés Illanes; Sonia E. Barberis (2024)

51 measurements across 1 enzyme  ·  doi:10.1007/s10930-024-10182-4

Computer-aided design to enhance the stability of aldo-keto reductase Kd AKR

Chen Dai; Jia-Xin Tian; Yu-Feng Chen; Yue-Han Ni; Lei Cui; Hai-Xing Cao; Lin-Lin Song; Shen-Yuan Xu; Ya-Jun Wang; Yu-Guo Zheng (2024)

Biotechnology Journal

18 measurements across 1 enzyme  ·  doi:10.1002/biot.202300637

Efficient biosynthesis of prunin in methanol cosolvent system by an organic solvent-tolerant α-L-rhamnosidase from Spirochaeta thermophila

Chen-Mu Luo; Li-Fan Ke; Xiang-Yu Huang; Xiao-Yan Zhuang; Ze-Wang Guo; Qiong Xiao; Jun Chen; Fu-Quan Chen; Qiu-Ming Yang; Yi Ru; Hui-Fen Weng; An-Feng Xiao; Yong-Hui Zhang (2024)

Enzyme and Microbial Technology

46 measurements across 1 enzyme  ·  doi:10.1016/j.enzmictec.2024.110410

Isolation of a Novel Low-Temperature-Active and Organic-Solvent-Stable Mannanase from the Intestinal Metagenome of Hermetia illucens

Dong-Gwan Kim; Chang-Muk Lee; Young-Seok Lee; Sang-Hong Yoon; Su-Yeon Kim (2024)

International Journal of Molecular Sciences

15 measurements across 1 enzyme  ·  doi:10.3390/ijms26010216

Expression optimization and characterization of a novel amylopullulanase from the thermophilic Cohnella sp. A01

Faezeh Hasani; Hossein Tarrahimofrad; Zohreh Javaheri Safa; Naser Farrokhi; Ali Asghar Karkhane; Kamahldin Haghbeen; Saeed Aminzadeh (2024)

International Journal of Biological Macromolecules

24 measurements across 1 enzyme  ·  doi:10.1016/j.ijbiomac.2024.135135

Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification

Frieda A. Sorgenfrei; Jeremy J. Sloan; Florian Weissensteiner; Marco Zechner; Niklas A. Mehner; Thomas L. Ellinghaus; Doreen Schachtschabel; Stefan Seemayer; Wolfgang Kroutil (2024)

Nature Communications

1493 measurements across 15 enzymes  ·  doi:10.1038/s41467-024-49774-0

Biocatalytic sulfation of aromatic and aliphatic alcohols catalyzed by arylsulfate sulfotransferases

Isabel Oroz-Guinea; Marko Rath; Isabelle Tischler; Klaus Ditrich; Doreen Schachtschabel; Michael Breuer; Wolfgang Kroutil (2024)

Applied Microbiology and Biotechnology

30 measurements across 5 enzymes  ·  doi:10.1007/s00253-024-13354-5

Characterization of a plant S-adenosylmethionine synthetase from Acacia koa

James T. Carrillo; Dulal Borthakur (2024)

Plant Physiology and Biochemistry

22 measurements across 1 enzyme  ·  doi:10.1016/j.plaphy.2024.108618

Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds

Jihene Maati; Jolanta Polak; Monika Janczarek; Marcin Grąz; Issam Smaali; Anna Jarosz-Wilkołazka (2024)

Biotechnology Letters

8 measurements across 1 enzyme  ·  doi:10.1007/s10529-024-03532-w