Pyruvate decarboxylase from Zymomonas mobilis
Enzyme Description
Sequence
MSYTVGTYLAERLVQIGLKHHFAVAGDYNLVLLDNLLLNKNMEQVYCCNELNCGFSAEGYARAKGAAAAVVTYSVGALSAFDAIGGAYAENLPVILISGAPNNNDHAAGHVLHHALGKTDYHYQLEMAKNITAAAEAIYTPEEAPAKIDHVIKTALREKKPVYLEIACNIASMPCAAPGPASALFNDEASDEASLNAAVEETLKFIANRDKVAVLVGSKLRAAGAEEAAVKFADALGGAVATMAAAKSFFPEENPHYIGTSWGEVSYPGVEKTMKEADAVIALAPVFNDYSTTGWTDIPDPKKLVLAEPRSVVVNGIRFPSVHLKDYLTRLAQKVSKKTGALDFFKSLNAGELKKAAPADPSAPLVNAEIARQVEALLTPNTTVIAETGDSWFNAQRMKLPNGARVEYEMQWGHIGWSVPAAFGYAVGAPERRNILMVGDGSFQLTAQEVAQMVRLKLPVIIFLINNYGYTIEVMIHDGPYNNIKNWDYAGLMEVFNGNGGYDSGAGKGLKAKTGGELAEAIKVALANTDGPTLIECFIGREDCTEELVKWGKRVAAANSRKPVNKLL
Bettina Rosche et al. (2004)
β Biphasic aqueous/organic biotransformation of acetaldehyde and benzaldehyde by Zymomonas mobilis pyruvate decarboxylase
Biotechnology and Bioengineering
Β· doi:10.1002/bit.20082 β
Β· Activity + Stability - Product Formation Stability - Half-life
▼
Database ID
UniProt: P06672 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli SG 13009 pPDC-WM
Experimental Data (24 measurements)
24 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | 1-Octanol | 50% | 0.75 | 8.9 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | 1-Heptanol | 50% | 0.75 | 9.6 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | 1-Hexanol | 50% | 0.75 | 11.4 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | 1-Pentanol | 50% | 0.75 | 10.8 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | Isoamyl Alcohol | 50% | 0.75 | 9.6 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | 1-Butanol | 50% | 0.75 | 10.1 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | Isobutanol | 50% | 0.75 | 10.8 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | n-Hexadecane | 50% | 0.75 | 0.3 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | Toluene | 50% | 0.75 | 2.0 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | Isobutyl Acetate | 50% | 0.75 | 3.0 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Activity + Stability - Product Formation | Product phenylacetylcarbinol formation after incubation (72 hours at 25Β°C) in the presence of organic solvent, measured by HPLC | tert-Butyl Methyl Ether (MTBE) | 50% | 0.75 | 4.0 | product concentration per unit enzyme | 50 mM MES/KOH, 5 mM MgSO4 | 7 | 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | 1mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in product concentration per unit enzyme) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | n-Hexadecane | 50% | 12.5 | 20.0 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | 1-Butanol | 50% | 12.5 | 8.0 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | n-Nonane | 50% | 12.5 | 16.5 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | n-Octane | 50% | 12.5 | 10.5 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | n-Heptane | 50% | 12.5 | 16.5 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | Methylcyclohexane | 50% | 12.5 | 10.0 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | 1-Nonanol | 50% | 12.5 | 11.5 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | n-Hexane | 50% | 12.5 | 6.7 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
| Stability - Half-life | Half-life (in days) in 25Β°C, with enzyme activity measured by HPLC in aqueous phase | 1-Octanol | 50% | 12.5 | 10.0 | hours | Incubation: 50 mM MES/KOH, 5 mM MgSO4, Assay: 50 mM MES/KOH, 5 mM MgSO4 | 7 | Incubation: 25Β°C, Assay: 25Β°C | 500 mM Acetaldehyde, 500 mM Benzaldehyde | Phenylacetylcarbinol | Incubation: 1 mM thiamine pyrophosphate, Assay: 1 mM thiamine pyrophosphate | Yes, "the stirrer speed was low enough to maintain separate phases" | Classical aqueous control (in hours) |
Visualization : Stability β Half-life
Bettina Rosche et al. (2004)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Activity + Stability β Product Formation
Bettina Rosche et al. (2004)
One bar per measurement. Colour = solvent, shade = solvent volume.
Samuel Sutiono et al. (2019)
β To beat the heatβengineering of the most thermostable pyruvate decarboxylase to date
RSC Advances
Β· doi:10.1039/c9ra06251c β
Β· Stability - Incubation
▼
Database ID
UniProt: P06672 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 4% | 100 | 99 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 8% | 100 | 98 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 12% | 100 | 97 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 16% | 100 | 89 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 20% | 100 | 57 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 24% | 100 | 11 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 28% | 100 | 7 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase | Ethanol | 32% | 100 | 6 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 50Β°C, Assay: 25Β°C | 25 mM Pyruvate | Acetaldehyde , Carbon Dioxide (CO2) | Incubation: 0.1 mM TDP, 5 mM MgSOβ, Assay: 0.1 mM TDP, 5 mM MgSOβ | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans |
Visualization : Stability β Incubation
Samuel Sutiono et al. (2019)
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.