Cytochrome P450 BM-3 from Bacillus megaterium

Enzyme Description

Species

Extremophile

EC Number

1.14.14.1 ↗

Sequence

Length: 1048 amino acids

TIKEMPQPKTFGELKNLPLLNTDKPVQALMKIADELGEIFKFEAPGRVTRYLSSQRLIKEACDESRFDKNLSQALKFVRDFAGDGLFTSWTHEKNWKKAHNILLPSFSQQAMKGYHAMMVDIAVQLVQKWERLNADEHIEVPEDMTRLTLDTIGLCGFNYRFNSFYRDQPHPFITSMVRALDEAMNKLQRANPDDPAYDENKRQFQEDIKVMNDLVDKIIADRKASGEQSDDLLTHMLNGKDPETGEPLDDENIRYQIITFLIAGHETTSGLLSFALYFLVKNPHVLQKAAEEAARVLVDPVPSYKQVKQLKYVGMVLNEALRLWPTAPAFSLYAKEDTVLGGEYPLEKGDELMVLIPQLHRDKTIWGDDVEEFRPERFENPSAIPQHAFKPFGNGQRACIGQQFALHEATLVLGMMLKHFDFEDHTNYELDIKETLTLKPEGFVVKAKSKKIPLGGIPSPSTEQSAKKVRKKAENAHNTPLLVLYGSNMGTAEGTARDLADIAMSKGFAPQVATLDSHAGNLPREGAVLIVTASYNGHPPDNAKQFVDWLDQASADEVKGVRYSVFGCGDKNWATTYQKVPAFIDETLAAKGAENIADRGEADASDDFEGTYEEWREHMWSDVAAYFNLDIENSEDNKSTLSLQFVDSAADMPLAKMHGAFSTNVVASKELQQPGSARSTRHLEIELPKEASYQEGDHLGVIPRNYEGIVNRVTARFGLDASQQIRLEAEEEKLAHLPLAKTVSVEELLQYVELQDPVTRTQLRAMAAKTVCPPHKVELEALLEKQAYKEQVLAKRLTMLELLEKYPACEMKFSEFIALLPSIRPRYYSISSSPRVDEKQASITVSVVSGEAWSGYGEYKGIASNYLAELQEGDTITCFISTPQSEFTLPKDPETPLIMVGPGTGVAPFRGFVQARKQLKEQGQSLGEAHLYFGCRSPHEDYLYQEELENAQSEGIITLHTAFSRMPNQPKTYVQHVMEQDGKKLIELLDQGAHFYICGDGSQMAPAVEATLMKSYADVHQVSEADARLWLQQLEEKGRYAKDVWAG

Tuck Seng Wong et al. (2003) — Laboratory evolution of cytochrome P450 BM-3 monooxygenase for organic cosolvents

Biotechnology and Bioengineering · doi:10.1002/bit.10896 ↗ · Activity - Classical

253 measurements ▼

Database ID

UniProt: P14779 ↗

Sequence Annotation

Inferred - from protein name (first residue from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli

Experimental Data (253 measurements)

253 measurements — page 13 of 13

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
F87A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	12.5%	100	11	4	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	15%	100	3	2	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	17.5%	100	0	1	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	100	0	0	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	1%	100	99	104	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	2.5%	100	101	103	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	5%	100	68	80	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	7.5%	100	50	68	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	37	34	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	12.5%	100	11	12	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	15%	100	3	7	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	17.5%	100	0	4	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)
F87A/T235A/R471A/E494K/S1024E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	100	0	2	%	?	?	Room temperature	0.5 mM p-Nitrophenoxylauric Acid	Hydroxylated fatty acid , p-Nitrophenol	? mM NADP+	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

—

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

—

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

—

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

—

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

—

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

—

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

7.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

10%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

12.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

15%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

1.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

7.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

12.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

15%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

7.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

10%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

2.5%

100

—

101

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

7.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

10%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

12.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

15%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

17.5%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

20%

100

—

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

108

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

115

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

107

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

111

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

117

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

118

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

118

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

112

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

112

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

119

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100

113

114

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1025

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100

110

130

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1026

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15%

100

106

152

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1027

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100

149

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1028

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25%

100

102

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1029

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024R

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/S1024T

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetone

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

101

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

1.5%

100

107

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

110

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

109

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

111

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

116

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

7.5%

100

108

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

1.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

1.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethylformamide (DMF)

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

112

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

113

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

108

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Acetonitrile

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

104

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

2.5%

100

101

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

107

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

7.5%

100

109

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

10%

100

106

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

17.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

2.5%

100

101

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

17.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

104

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

2.5%

100

101

103

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

7.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

10%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

12.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

15%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

17.5%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

F87A/T235A/R471A/E494K/S1024E

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Ethanol

20%

100

Room temperature

0.5 mM p-Nitrophenoxylauric Acid

Hydroxylated fatty acid, p-Nitrophenol

? mM NADP+

—

Classical aqueous control (in %)

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Mutations in this dataset (16)

WT F87A F87A/T235A/R471A/E494K/S1024E F87A/T235A/R471A/S1024R F87A/T235A/R471A/S1024T F87A/T235A/S1024R T235A/R471A/E494K/S1024 T235A/R471A/E494K/S1024E T235A/R471A/E494K/S1025 T235A/R471A/E494K/S1026 T235A/R471A/E494K/S1027 T235A/R471A/E494K/S1028 T235A/R471A/E494K/S1029 T235A/R471A/S1024R T235A/R471A/S1024T T235A/S1024R

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Cytochrome P450 BM-3 from Bacillus megaterium

Enzyme Description

Sequence

Experimental Data (253 measurements)

Visualization : Activity — Classical

Mutation Effect

Structure

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