Lipase from Rhizomucor miehei

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 363 amino acids

MVLKQRANYLGFLIVFFTAFLVEAVPIKRQSNSTVDSLPPLIPSRTSAPSSSPSTTDPEAPAMSRNGPLPSDVETKYGMALNATSYPDSVVQAMSIDGGIRAATSQEINELTYYTTLSANSYCRTVIPGATWDCIHCDATEDLKIIKTWSTLIYDTNAMVARGDSEKTIYIVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQNELVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVSTGIPYRRTVNERDIVPHLPPAAFGFLHAGEEYWITDNSPETVQVCTSDLETSDCSNSIVPFTSVLDHLSYFGINTGLCT

Wakako TSUZUKI et al. (2003) — Polar Organic Solvent Added to an Aqueous Solution Changes Hydrolytic Property of Lipase

Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.67.1660 ↗ · Activity - Classical Stability - Incubation

190 measurements ▼

Database ID

UniProt: P19515 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (190 measurements)

190 measurements — page 8 of 10

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	10%	100.0	62.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	20%	100.0	55.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	30%	100.0	4.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	10%	100.0	20.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	20%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	10%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	20%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100.0	103.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	109.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100.0	150.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100.0	186.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	25%	100.0	175.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100.0	96.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	35%	100.0	56.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100.0	44.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	45%	100.0	39.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100.0	34.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	60%	100.0	28.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	70%	100.0	13.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	80%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	90%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	97.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	96.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	98.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	103.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100.0	139.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	175.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	35%	100.0	200.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100.0	178.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	45%	100.0	120.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	81.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100.0	52.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	70%	100.0	39.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	80%	100.0	21.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	90%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	2.5%	100.0	90.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	5%	100.0	87.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	7.5%	100.0	103.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	10%	100.0	134.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	12.5%	100.0	141.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	15%	100.0	142.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	17.5%	100.0	145.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	20%	100.0	133.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	22.5%	100.0	107.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	25%	100.0	75.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	30%	100.0	15.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	35%	100.0	3.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	45%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	5%	100.0	87.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	10%	100.0	65.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	15%	100.0	60.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	20%	100.0	51.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	25%	100.0	1.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	35%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethoxymethane	45%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	2%	100.0	118.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	4%	100.0	111.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	5%	100.0	103.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	7.5%	100.0	79.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	10%	100.0	68.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	12.5%	100.0	33.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	15%	100.0	7.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	17.5%	100.0	2.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	20%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	25%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	35%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	45%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Diethoxyethane	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	2%	100.0	86.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	4%	100.0	65.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	6%	100.0	57.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	8%	100.0	54.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	10%	100.0	44.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	12%	100.0	28.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	14%	100.0	13.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	16%	100.0	3.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	20%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	25%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	35%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	2,2-Dimethoxypropane	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	2.5%	100.0	8.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	5%	100.0	1.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	10%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	15%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	20%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	25%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	35%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	45%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Anisole	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	5%	100.0	71.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100.0	45.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	15%	100.0	34.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100.0	26.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	25%	100.0	23.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30%	100.0	19.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	35%	100.0	21.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	40%	100.0	24.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	45%	100.0	25.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	50%	100.0	25.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	60%	100.0	21.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	70%	100.0	7.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	80%	100.0	1.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	90%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	5%	100.0	85.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	10%	100.0	97.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	15%	100.0	185.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	20%	100.0	164.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	25%	100.0	103.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	30%	100.0	39.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	35%	100.0	16.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	40%	100.0	4.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	45%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	80%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	90%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	5%	100.0	50.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	10%	100.0	25.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	15%	100.0	14.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	20%	100.0	6.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	25%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	35%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	45%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	80%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,3-Dioxane	90%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	10%	100.0	67.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	20%	100.0	46.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	30%	100.0	36.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	40%	100.0	7.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Methanol	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	10%	100.0	62.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	20%	100.0	55.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	30%	100.0	4.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Ethanol	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	10%	100.0	20.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	20%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Propanol	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	10%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	20%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	30%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	40%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	50%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	60%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1-Butanol	70%	100.0	0.0	%	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	4.5	8.2	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	4.5	9.1	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	15%	4.5	8.3	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	15%	4.5	6.3	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	4.6	9.3	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl palmitate	4-Methylumbelliferone, Palmitic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	4.6	9.7	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl palmitate	4-Methylumbelliferone, Palmitic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	15%	4.6	8.2	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl palmitate	4-Methylumbelliferone, Palmitic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	15%	4.6	6.7	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl palmitate	4-Methylumbelliferone, Palmitic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	3.9	1.8	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl nonanoate	4-Methylumbelliferone, Nonanoic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	3.9	1.6	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl nonanoate	4-Methylumbelliferone, Nonanoic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	15%	3.9	1.8	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl nonanoate	4-Methylumbelliferone, Nonanoic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	15%	3.9	3.0	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl nonanoate	4-Methylumbelliferone, Nonanoic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	3.5	0.1	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl heptanoate	4-Methylumbelliferone, Heptanoic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	3.5	0.15	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl heptanoate	4-Methylumbelliferone, Heptanoic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	15%	3.5	0.2	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl heptanoate	4-Methylumbelliferone, Heptanoic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	15%	3.5	0.75	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl heptanoate	4-Methylumbelliferone, Heptanoic Acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	4.4	5.9	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 1-Monocaprin	Capric acid, glycerol	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Initial reaction rate (mmol/(min*mg) measured by fluorometry	Dimethyl Sulfoxide (DMSO)	35%	4.4	6.0	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 1-Monocaprin	Capric acid, glycerol	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Initial reaction rate (mmol/(min*mg) measured by fluorometry	Dimethylformamide (DMF)	20%	1.4	1.8	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 2-Monocaprin	Capric acid, glycerol	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Initial reaction rate (mmol/(min*mg) measured by fluorometry	Dimethyl Sulfoxide (DMSO)	35%	1.4	2.0	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 2-Monocaprin	Capric acid, glycerol	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Initial reaction rate (mmol/(min*mg) measured by fluorometry	Dimethylformamide (DMF)	20%	2.7	4.4	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 1-Monoolein	glycerol, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Initial reaction rate (mmol/(min*mg) measured by fluorometry	Dimethyl Sulfoxide (DMSO)	35%	2.7	5.4	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 1-Monoolein	glycerol, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Initial reaction rate (mmol/(min*mg) measured by fluorometry	Dimethylformamide (DMF)	20%	0.9	1.3	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 2-Monoolein	glycerol, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Activity - Classical	Initial reaction rate (mmol/(min*mg) measured by fluorometry	Dimethyl Sulfoxide (DMSO)	35%	0.9	1.6	mmol/(min*mg)	0.04M Britton-Robinson buffer	7	37°C	? mM 2-Monoolein	glycerol, Oleic acid	—	—	Classical aqueous control (in mmol/(min*mg))
Stability - Incubation	Activity after incubation (6 days at 37°C) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	95.0	93.0	%	Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer	Incubation: 7, Assay: 7	Incubation: 37°C, Assay: 37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 days at 37°C) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,4-Dioxane	30%	95.0	91.0	%	Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer	Incubation: 7, Assay: 7	Incubation: 37°C, Assay: 37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 days at 37°C) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	1,2-Dimethoxyethane	15%	95.0	91.0	%	Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer	Incubation: 7, Assay: 7	Incubation: 37°C, Assay: 37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 days at 37°C) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	95.0	88.0	%	Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer	Incubation: 7, Assay: 7	Incubation: 37°C, Assay: 37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone, Oleic acid	—	—	Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in the presence of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation