Lactoperoxidase from Bubalus bubalis (domestic water buffalo)

Enzyme Description

Species

Bubalus bubalis (domestic water buffalo) ↗

Extremophile

EC Number

1.11.1.7 ↗

Sequence

Length: 712 amino acids

MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGQTRTAIRNGQVWEESFKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLTRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKFSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN

H. Özdemir et al. (2003) — Effects of the Water-Miscible Organic Solvents on Lactoperoxidase Purified from Creek-Water Buffalo Milk

Biochemistry (Moscow) · doi:10.1023/a:1023062402300 ↗ · Activity - Classical

39 measurements ▼

Database ID

UniProt: A5JUY8 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, eukaryote Bubalus bubalis (domestic water buffalo)

Experimental Data (39 measurements)

39 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.50M	100	57	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	1.3M	100	50	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	1.9M	100	34	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	3.8M	100	8	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	0.4M	100	72	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	0.8M	100	50	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	1.25M	100	41	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	2.5M	100	16	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	3.3M	100	4	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.25M	100	76	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	0.65M	100	74	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.7M	100	40	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1.4M	100	13	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2.8M	100	1	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.2M	100	70	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.4M	100	46	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.65M	100	28	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1.3M	100	2	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethylene Glycol	0.6M	100	95	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	0.6M	100	62	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethylene Glycol	1.8M	100	82	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethylene Glycol	3.5M	100	54	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethylene Glycol	4.7M	100	37	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethylene Glycol	6.5M	100	15	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	0.8M	100	87	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	1.6M	100	73	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	2.5M	100	60	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	5M	100	15	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	6.5M	100	5	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethylene Glycol	1.2M	100	90	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	1.15M	100	42	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	1.75M	100	29	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	3.5M	100	6	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	4.6M	100	1	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.2M	100	69	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.4M	100	49	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.65M	100	42	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	1.25M	100	20	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	2.5M	100	3	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.50M	100	57	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	1.3M	100	50	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	1.9M	100	34	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	3.8M	100	8	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	0.4M	100	72	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	0.8M	100	50	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	1.25M	100	41	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	2.5M	100	16	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	3.3M	100	4	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.25M	100	76	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	0.65M	100	74	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.7M	100	40	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1.4M	100	13	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2.8M	100	1	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.2M	100	70	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.4M	100	46	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.65M	100	28	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1.3M	100	2	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent	Ethylene Glycol	0.6M	100	95	%	100 mM Phosphate buffer	6.8	25°C	1 mM ABTS, 0.1 mM H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	—	—	Classical aqueous control (in %)

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