Transketolase (gene tktA) from Escherichia coli

Enzyme Description

Species
Escherichia coli β†—
Extremophile
No
EC Number
2.2.1.1 β†—

Sequence

Length: 663 amino acids
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL
Phattaraporn Morris et al. (2021) β€” Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents
Scientific Reports  Β· doi:10.1038/s41598-021-03001-8 β†—  Β· Stability - Incubation
71 measurements ▼
Database ID
UniProt: P27302 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli XL10-Gold

Experimental Data (71 measurements)

71 measurements β€” page 2 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 8% 19.3 0.0 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 6% 19.3 0.3 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 4% 19.3 0.4 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 2% 19.3 0.4 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 1% 19.3 1.1 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 0.5% 19.3 5.4 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 10% 19.3 0.0 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Ethyl Acetate 10% 19.3 3.0 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 6% 19.3 0.3 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 4% 19.3 0.4 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 2% 19.3 6.2 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 1% 19.3 15.8 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 0.5% 19.3 17.8 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 25% 19.3 0.0 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 20% 19.3 5.4 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 15% 19.3 10.1 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Acetonitrile 2% 19.3 16.2 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Acetonitrile 30% 19.3 0.0 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Acetonitrile 25% 19.3 0.0 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Acetonitrile 20% 19.3 0.0 Β΅M/mg/mL Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
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Visualization : Stability β€” Incubation

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Structure

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