Cholinesterase (gene BCHE) from Homo sapiens

Enzyme Description

Species

Extremophile

EC Number

3.1.1.8 ↗

Sequence

Length: 529 amino acids

EDDIIIATKNGKVRGMQLTVFGGTVTAFLGIPYAQPPLGRLRFKKPQSLTKWSDIWNATKYANSCCQNIDQSFPGFHGSEMWNPNTDLSEDCLYLNVWIPAPKPKNATVLIWIYGGGFQTGTSSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNMGLFDQQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEILLNEAFVVPYGTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFHYTDWVDDQRPENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLERRDQYTKAEEILSRSIVKRWANFAKYGNPQETQNQSTSWPVFKSTEQKYLTLNTESTRIMTKLRAQQCRFWTSFFPKV

Noelle A. Novales et al. (2022) — Comparing the effects of organic cosolvents on acetylcholinesterase and butyrylcholinesterase activity

Analytical Biochemistry · doi:10.1016/j.ab.2022.114796 ↗ · Activity - Classical Activity - Michaelis-Menten

28 measurements ▼

Database ID

UniProt: P06276 ↗

Sequence Annotation

Explicit - Provided PDB Accession Number (28 first residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (28 measurements)

28 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	0.125	0.33	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	0.125	0.245	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	0.125	0.14	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	0.125	0.16	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	120.0	120.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	120.0	119.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	120.0	116.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	120.0	132.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Methanol	2%	100.0	103.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Methanol	1%	100.0	102.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Dimethyl Sulfoxide (DMSO)	5%	100.0	39.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Dimethyl Sulfoxide (DMSO)	2%	100.0	64.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Dimethyl Sulfoxide (DMSO)	1%	100.0	82.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetone	5%	100.0	30.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetone	2%	100.0	66.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetone	1%	100.0	86.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetonitrile	5%	100.0	73.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetonitrile	2%	100.0	99.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetonitrile	1%	100.0	101.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Methanol	5%	100.0	106.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	0.125	0.5	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	5%	0.125	0.56	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	5%	0.125	0.225	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	5%	0.125	0.241	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	120.0	116.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	5%	120.0	100.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	5%	120.0	112.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	5%	120.0	135.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	0.125	0.33	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	0.125	0.245	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	0.125	0.14	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	0.125	0.16	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	120.0	120.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	120.0	119.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	120.0	116.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	120.0	132.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Butyrylthiocholine	butyrate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten