Acetylcholinesterase (gene Ache) from Mus musculus (mouse)

Enzyme Description

Species

Extremophile

EC Number

3.1.1.7 ↗

Sequence

Length: 540 amino acids

EDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSAT

Noelle A. Novales et al. (2022) — Comparing the effects of organic cosolvents on acetylcholinesterase and butyrylcholinesterase activity

Analytical Biochemistry · doi:10.1016/j.ab.2022.114796 ↗ · Activity - Classical Activity - Michaelis-Menten

28 measurements ▼

Database ID

UniProt: P21836 ↗

Sequence Annotation

Explicit - Provided PDB Accession Number (34 first residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (28 measurements)

28 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	0.076	0.622	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	0.076	0.525	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	0.076	0.175	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	0.076	0.1	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	133.0	82.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	133.0	105.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	133.0	114.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	133.0	138.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate , Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Methanol	2%	100.0	92.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Methanol	1%	100.0	95.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Dimethyl Sulfoxide (DMSO)	5%	100.0	5.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Dimethyl Sulfoxide (DMSO)	2%	100.0	14.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Dimethyl Sulfoxide (DMSO)	1%	100.0	25.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetone	5%	100.0	12.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetone	2%	100.0	23.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetone	1%	100.0	41.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetonitrile	5%	100.0	21.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetonitrile	2%	100.0	50.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Acetonitrile	1%	100.0	67.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm)	Methanol	5%	100.0	81.0	%	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in %)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	0.076	1.65	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	5%	0.076	1.35	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	5%	0.076	0.497	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	5%	0.076	0.155	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	133.0	58.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	5%	133.0	78.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	5%	133.0	91.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	5%	133.0	135.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	0.076	0.622	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	0.076	0.525	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	0.076	0.175	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	0.076	0.1	mM	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	133.0	82.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetone	2%	133.0	105.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Acetonitrile	2%	133.0	114.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)
Activity - Michaelis-Menten	Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent	Methanol	2%	133.0	138.0	10^-8 * mol/s	100 mM sodium phosphate buffer, 1 mM MgCl₂, 0.2 MM Ellman's reagent	7.5	25°C	100 µM Acetylthiocholine	Acetate, Thiocholine	—	—	Classical aqueous control (in 10^-8 * mol/s)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten