5'-nucleotidase from Shewanella amazonensis

Enzyme Description

Species

Extremophile

EC Number

3.1.3.5 ↗

Sequence

Length: 569 amino acids

MTNKLIKGLIATAVLAALAGCNSDDDKVPTTCAEAGAACKTFTVLHTNDNHGRFWENKDGEYGLAAQKTLVDQIRAEVSKKGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSIVDMQRSLAEFPMLAANIYRKNAKGEPGERYFDAYKIFDVNGIKVAVIGLTTEDTKKIGNPEYISELFFTDPKTEVAKVIKEIKDAKSADIIFAVTHMGHYADGQNGSNAPGDVALARSLKEGELQVVIGGHSQNPVCMEPGNKAYAAFQPGDACAPDQQNGTWIMQAHEWGKYVGRADFEYFNGELHLASYKLVVPNLVKEVTDEAGNKKKLVVGEKIEPDTELKELLSYYQARGQGQLDEEIADTDGVLEKSRLKQTNLGRLIADAQRTKVGADIGIMNSGGVRASIETGKITYRDVLTVQPFGNMVTLSTKTGAELTEYLGAVASLQIGAGAYAQITGVKMNVDCIAKTVNISEVNGKPFSAINNYTFTVPSFNAAGGDGYPKLNPVQTGYVDAEVLYTFFKNKNQIVAADYNPVGDIVYENSESVEGCKITAK

Kiko Fujimori et al. (2019) — Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions

Bioscience, Biotechnology, and Biochemistry · doi:10.1080/09168451.2019.1578641 ↗ · Activity - Classical

24 measurements ▼

Database ID

UniParc: UPI0004D6B912 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Purified, bacterium Shewanella amazonensis

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	96.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	116.0	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	30%	100.0	8.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	20%	100.0	36.0	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Ethanol	10%	100.0	97.6	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetone	30%	100.0	30.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetone	20%	100.0	58.8	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetone	10%	100.0	90.8	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	16.3	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	53.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	85.7	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Isopropanol	30%	100.0	17.7	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Isopropanol	20%	100.0	55.8	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Isopropanol	10%	100.0	90.8	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Ethanol	30%	100.0	33.7	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Ethanol	20%	100.0	69.0	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	10%	100.0	96.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Methanol	30%	100.0	43.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Methanol	20%	100.0	60.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Methanol	10%	100.0	83.3	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100.0	52.0	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100.0	77.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	105.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	74.1	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	96.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	116.0	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	30%	100.0	8.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	20%	100.0	36.0	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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Structure

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