5'-methylthioadenosine phosphorylase from Pyrococcus furiosus

Enzyme Description

Species

Pyrococcus furiosus ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

2.4.2.28 ↗

Sequence

Length: 257 amino acids

MPKIGIIGGSGVYGIFEPKETVKVHTPYGRPSAPVEIGEIEGVEVAFIPRHGKYHEFPPHEVPYRANIWALHELGVERVIAVNAVGSLKEEYKPGDIVIIDQFIDFTKKREYTFYNGPRVAHISMADPFCPELRRIFIETAKELNLPVHEKGTYICIEGPRFSTRAESRMFRQFADVIGMTLVPEVNLARELGMCYVNISTVTDYDVWAEKPVDAQEVLRVMKENEEKVQKLLKRAIPKIPEERKCGCADVLKTMFV

Giovanna Cacciapuoti et al. (2003) — Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus

Extremophiles · doi:10.1007/s00792-002-0307-2 ↗ · Stability - Incubation

36 measurements ▼

Database ID

UniProt: Q8U4Q8 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Purified, archaeon Pyrococcus furiosus

Experimental Data (36 measurements)

36 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	90	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	92	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	77	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	48	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	90	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	72	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	77	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	10	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	71	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	62	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	62	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate , Adenine	—	—	Non-incubated control (in %), assay in aqueous phase

Stability - Incubation	Activity after incubation (30 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	29	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	65	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	58	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	50	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	37	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	5	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	2	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	23	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	30	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	22	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	38	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 90°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	3	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 90°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	88	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	90	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	92	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	77	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	48	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	90	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	72	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	77	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 50°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Dimethylformamide (DMF)	50%	100	10	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Methanol	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Ethanol	50%	100	71	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	1-Propanol	50%	100	62	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Acetonitrile	50%	100	100	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 70°C) using radiochemistry (reaction product [methyl-14C]5-methylthioribose-1-phosphate radioactive counts measurement) in aqueous phase	Tetrahydrofuran (THF)	50%	100	62	%	Incubation: 100 mM Tris-HCl, Assay: 20 µM phosphate buffer	Incubation: 7.4, Assay: 4	Incubation: 70°C, Assay: 80°C	0.4 mM 5'-[methyl-14C]thioadenosine	[methyl-14C]5-methylthioribose-1-phosphate, Adenine	—	—	Non-incubated control (in %), assay in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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