β-trypsin from Bos taurus (bovine)

Enzyme Description

Species

Extremophile

EC Number

3.4.21.4 ↗

Sequence

Length: 223 amino acids

IVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN

Dayanne Pinho Rosa et al. (2021) — Evaluation of biological activities, structural and conformational properties of bovine beta- and alpha-trypsin isoforms in aqueous-organic media

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.02.079 ↗ · Activity - Classical Stability - Tm

33 measurements ▼

Database ID

UniProt: P00760 ↗

Sequence Annotation

Inferred - from protein name (23 first residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (33 measurements)

33 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	70%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	80%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	90%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	46.0	51.6	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	46.0	56.5	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	46.0	55.5	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	40%	46.0	54.9	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	60%	46.0	3.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine , p-Nitroaniline	—	—	Classical aqueous control (in microM/L)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	20%	56.5	43.2	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	40%	56.5	48.8	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	50%	56.5	48.2	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	60%	56.5	53.4	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	80%	56.5	68.6	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	20%	46.0	37.4	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	90%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	80%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	70%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	60%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	50%	46.0	1.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	40%	46.0	3.9	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	32%	46.0	25.3	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	30%	46.0	33.2	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	20%	46.0	47.7	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	10%	46.0	50.2	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	90%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	80%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	70%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	50%	46.0	28.8	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	46.0	38.6	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	30%	46.0	37.7	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	40%	46.0	41.1	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	50%	46.0	21.2	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	60%	46.0	4.7	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	70%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	80%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	90%	46.0	0.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	46.0	51.6	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	46.0	56.5	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	46.0	55.5	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	40%	46.0	54.9	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	60%	46.0	3.0	µM/L	100 mM Tris-HCl buffer, 20 mM CaCl2	8	37°C	0.9 mM Benzoyl-DL-arginine p-nitroanilide	N-Benzoyl-L-arginine, p-Nitroaniline	—	—	Classical aqueous control (in µM/L)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	20%	56.5	43.2	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	40%	56.5	48.8	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	50%	56.5	48.2	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	60%	56.5	53.4	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)
Stability - Tm	Tm measured by UV-absorption spectroscopy in the presence of organic solvent	Ethanol	80%	56.5	68.6	°C	50 mM glycine buffer, 20 mM CaCl2	3	—	—	—	—	—	Classical aqueous control (in °C)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — T_m

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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