S-adenosylmethionine from Acacia koa

Enzyme Description

Species

Extremophile

EC Number

2.5.1.6 ↗

Sequence

Length: 393 amino acids

METFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCLVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISIHLDLKRGGNGRFLKTAAYGHFGREDPDFTWETVKPLKWEKPQA

James T. Carrillo et al. (2024) — Characterization of a plant S-adenosylmethionine synthetase from Acacia koa

Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2024.108618 ↗ · Activity - Michaelis-Menten Activity + Stability - Product Formation

22 measurements ▼

Database ID

UniProt: A0A9E8G339 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Methanol	10%	1.0	0.8	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi , PPi , S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Methanol	5%	1.0	0.6	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi , PPi , S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)

Activity - Michaelis-Menten	kcat (1/s) measured by HPLC in the presence of organic solvent	Methanol	25%	1.69	1.86	1/s	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (1/s) measured by HPLC in the presence of organic solvent	Formamide	25%	1.69	1.51	1/s	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (1/s) measured by HPLC in the presence of organic solvent	Acetonitrile	25%	1.69	1.77	1/s	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in 1/s)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	1.0	0.12	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	1.0	0.54	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	1.0	0.49	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	1.0	0.58	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	50%	1.0	0.24	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	25%	1.0	1.36	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	10%	1.0	0.76	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	5%	1.0	0.58	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	50%	1.0	0.1	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	25%	1.0	0.24	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	10%	1.0	0.37	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Formamide	5%	1.0	0.56	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Acetonitrile	25%	1.0	1.3	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Acetonitrile	10%	1.0	0.8	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Acetonitrile	5%	1.0	0.6	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Methanol	50%	1.0	0.7	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Methanol	25%	1.0	1.6	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Methanol	10%	1.0	0.8	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)
Activity + Stability - Product Formation	Relative product formation after 1 hour incubation at 35°C, measured by HPLC in the presence of organic solvent	Methanol	5%	1.0	0.6	relative units, 1 for control	100 mM Tris-HCl buffer, 20 mM KCl, 10 mM MgCl₂, 5 mM DTT	8.5	35°C	ATP (Adenosine triphosphate), 4 mM L-methionine	Pi, PPi, S-adenosyl-L-methionine	—	—	Classical aqueous control (in relative units, 1 for control)

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Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability — Product Formation