Amylopullulanase (gene Amy1136) from Cohnella sp. A01

Enzyme Description

Species

Cohnella sp. A01 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.2.1.41 ↗, 3.2.1.1 ↗

Sequence

Length: 721 amino acids

MSLKKKVTAGTLAASLAMTALAGIPLSSKGLAQKLGLNNIASAASISGSQLGFFASQLKSALSASGAVYTKSVADSVYAYFDSVSSVTYVTYDGVTTPTSDIIKPVKDKIKFKEDSGLQLTSEDEKNLELVFATLAKFTFELEAGKLDALQSNESVRATLRKIAQGAGVDGLTVEDLNGLLFGAKGIEAIAWQELANSSQSLPDFLNNDEARNDFVKRVFKAYLNTNEDLAKALKAYEVNEEDLARVVSNFRDAVGASNFNKAAAALVEAYINVKSSPVSGGGSGVSNNVLDNARKELAAIKAKLASASDAEKQALIDQALKIAAEAIAKLAQFDASSLVKEVDGKAVVSLSLANLSSLFSSVEQLKAALADAIGADNAAKLAKPTLVIDLGKTTLKSSVVNLAKDISAKAKELNFGWIAIKVSGATTKFPLSTLGGDVIYTIDRTAASASQTGGRPAVTDVFNYSLSVNGTVYDKFNEPVEIQLPVTNTSGVDTELLSVAKLDNGSLSIEGGVYGDGVLTEQRYTFSSYVGVENDTFRDVASVQAWAGRQIKVVAAKGAIEGRGDGRFDPRANVTRAEFAKILVSALDLDNPNVTSTKFTDVNPNAWYAPYVAVAAEKGIINGRSATKFDPNAPISRAEIATLIARALQVTKGFSEVKDIDAALANFSDAGKIPSSLKAGVAFAASKNIVIGTNGKFNPNDNASRAEAAVIVYRALNAKA

Faezeh Hasani et al. (2024) — Expression optimization and characterization of a novel amylopullulanase from the thermophilic Cohnella sp. A01

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.135135 ↗ · Activity - Classical

24 measurements ▼

Database ID

—

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	1%	100	51	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	2%	100	49	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	1%	100	82	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	2%	100	55	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	1%	100	54	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	2%	100	58	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	1%	100	79	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	2%	100	21	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	1%	100	55	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	2%	100	76	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	1%	100	69	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	2%	100	91	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	1%	100	70	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	2%	100	94	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	1%	100	76	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	2%	100	54	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	1%	100	41	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	2%	100	62	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	1%	100	49	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	2%	100	62	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	1%	100	51	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	2%	100	49	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	1%	100	82	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	2%	100	55	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Methanol	1%	100	54	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	2%	100	58	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	1%	100	79	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	2%	100	21	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Acetone	1%	100	55	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	2%	100	76	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	1%	100	69	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Ethanol	2%	100	91	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	1%	100	70	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	2%	100	94	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	1%	100	76	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	2%	100	54	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isobutanol	1%	100	41	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	2%	100	62	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	1%	100	49	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	2%	100	62	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	1%	100	66	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	2%	100	57	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	1%	100	88	%	50 mM potassium-phosphate buffer	8	50°C	1% Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	2%	100	48	%	50 mM potassium-phosphate buffer	8	50°C	1% Starch	Reducing sugars	—	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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Structure

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