α-L-rhamnosidase from Dictyoglomus thermophilum

Enzyme Description

Species

Dictyoglomus thermophilum ↗

Extremophile

Yes thermophilic organism

EC Number

3.2.1.40 ↗

Sequence

Length: 921 amino acids

MKSSNIYSPFDLKCEFTTNPLGVDKKNPIFSWKLRHLEKNEKQTAYQVIVSSSLETINDNIGDVWDTGKVLSSEQVIKYEGKELEPCKVYFWKVRWWDSKDQESPFSVVNTFETGLMNEENWKAKWITKKEHKYEVYSPDGAPFGLNYTIAYAPMFRKSFSISKKIKRARVYIAGLGLYELYINGERIGDRVLDPGQTDYKKRVLYTVYDVSKNIRDGKNAIGVILGNGRYVKEYGYDFPKLIIQVLVEYEDDSIEWIVSDESWKTTYGPITLNSLYHGEIYDGRKEIKGWNLPDFDDSTWENAILAEPPGGKLYSEIYPPIRITKTIKPIKMWSPEPGTYVYDFGQNYTGWIKIKVRTNESGKEIRIRHAELTYEDGTLNYSTNRTALATDVYITKGEGYEEYEPRFTYHGFRYVEILGYPGVPTLEDIEGKVVHTAVESNGEFICSNELINKIHHNIIWGQLSNLMSIPTDCPQRDERMGWMGDAQLSAEEAIFNFDMIGFYRKYLNDIRDAQKENGSLSDVIPPYWSIYPGDPAWSTAYITIAWYLYQYYGDKYVLEEHYEGFKKYVEFLKKLAPDYIVSFYKYGDWCQPGTVRPKDNSGELTSTFYFYHDVITLSKIAKLLGKEADYKYYSELADKIKSAFNKKFLKEKAYASIPTELSEENVKALLEKYPEDIKDFLRQQFTILSSLGMFTSQTLNTLPLYLNLVPEDKVQDVLKTLLEDIIIRHDYHLDTGIVATRYIFDVLTSYGYDEVAYKIVNQKTYPSFGYMIEEGATTLWERWEKLTSTGMNSHNHIMFGSVDAWFYRVIAGVRVGEPGWNKIIFEPHPVGDLKYAKARLNTIKGEVEINWQKTENIFSMRISVPVNSEGEVHVPKLFERFVVKEGDNIIYEKKGDLEENEKYIVIRVGSGSYNFYMEKI

Jinyue Hu et al. (2025) — An Organic Solvent-Tolerant α-L-Rhamnosidase from Dictyoglomus thermophilum and Its Application in Production of Icariside I from Icariin

Molecules · doi:10.3390/molecules30132847 ↗ · Activity - Classical Stability - Incubation Activity + Stability - Conversion

57 measurements ▼

Database ID

UniProt: B5YC64 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (57 measurements)

57 measurements — page 3 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	62	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	87	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	5	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	131	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	112	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	87	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	65	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	53	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	100	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	68	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	43	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	26	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	11	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	67	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	36	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	18	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	10	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	144	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100	96	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	113	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	121	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	188	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	40%	100	72	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	20%	100	90	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100	118	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	40%	100	97	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	161	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100	102	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	40%	100	102	%	100 mM sodium acetate buffer	6	55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (2 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	5%	50	75	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (1 hour at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	5%	28	36	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (4 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	5%	62	90	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (6 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	5%	63	91	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (8 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	5%	66	91	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (1 hour at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	10%	28	50	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (2 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	10%	50	90	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (4 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	10%	62	96	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (6 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	10%	63	97	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (8 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	10%	66	97	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (1 hour at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	20%	28	30	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (2 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	20%	50	46	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (4 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	20%	62	55	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (6 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	20%	63	58	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion after incubation (8 hours at 55°C) in the presence of organic solvent, measured by HPLC (icariin concentration)	Dimethyl Sulfoxide (DMSO)	20%	66	58	%	100 mM sodium acetate buffer	6	55°C	3 mM Icariin	icariside I	—	180 rpm	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	96	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100	38	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100	53	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100	70	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100	94	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100	122	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	23	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	35	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	56	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	73	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	91	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	19	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	34	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	62	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	87	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	5	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	131	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	112	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	87	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	65	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	53	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	100	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	68	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	43	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	26	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (4 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	11	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	67	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	36	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	18	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	10	%	Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer	Incubation: 6, Assay: 6	Incubation: 55°C, Assay: 55°C	3 mM p-Nitrophenylrhamnoside	L-Rhamnose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay really was in the presence of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability — Conversion

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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α-L-rhamnosidase from Dictyoglomus thermophilum

Enzyme Description

Sequence

Experimental Data (57 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Visualization : Activity + Stability — Conversion

Structure

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