Chitosinase (gene ChoK) from Bacillus sp. CK4

Enzyme Description

Species
Bacillus sp. CK4 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.132 β†—

Sequence

Length: 273 amino acids
MREADWWGKAAISLLVFTMFFTLMMSETVFAAGLNKDQKRRAEQLRRICEDGTTEMRYPYVARLDDARPSTCGPAGVTTATRDGFEVVPVYKQAVANKKLPNYLAGLRRLEKEASDDTSKLKGPASAWKSLADDKAFRAAQDGVNDQVYYQPAMERSDNAGLTTALARAVMYHTVRQRGDGDDGDSRYALIKRTPKGAGGSPKEGIDEQKCLNKFSHVRYDDLMNGANHDRRDEWRESVGRVHVLRSIANQNNYNLNGGIHVRSHEYGNGVIK
Ho-Geun YOON et al. (2001) β€” Thermostable Chitosanase from Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1271/bbb.65.802 β†—  Β· Stability - Incubation
30 measurements ▼
Database ID
UniProt: Q9RC18 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Bacillus sp. CK4

Experimental Data (30 measurements)

30 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 10% 100 90 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 50% 100 20 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 40% 100 47 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 30% 100 71 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 20% 100 89 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 10% 100 99 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 50% 100 30 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 40% 100 50 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 30% 100 66 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 20% 100 82 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 10% 100 99 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 50% 100 10 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 40% 100 33 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 30% 100 55 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 20% 100 76 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethanol 10% 100 100 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 50% 100 43 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 40% 100 65 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 30% 100 83 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 20% 100 92 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

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Structure

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