β-xylosidase Dt-2286 from Dictyoglomus turgidum

Enzyme Description

Species

Dictyoglomus turgidum DSM6724 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.2.1.37 ↗

Sequence

Length: 762 amino acids

MEEKEILKKVRDLISKMTLEEKIAQLQSVFGKELVDESGNFSEEKAEKLLKNGIGQISRVAGEKGMDPERAVELANKIQKFLKEKTRLGIPAIIHEECLSGFMAKGATVFPQAIGMASTFEPELIRRVSDVIRQHMRAANVHQGLSPVLDIPRDPRWGRTEETFGEDPYLVSRMAAEYVKGLQGEDWREGIIATVKHFTAYGISEGARNLGPAKVGERELREVFLFPFEVAIKEGQAGSLMNAYHEIDGVPCASSKFLLTKILRWEWGFKGYVVSDYIAIRMLENFHRVAKDAKEAAVLALEAGIDIELPSVDCYGEPLIQAVKEGLISEEVINASVERVLRAKFMLGLFDGDLEKDPKKVYDIFDKPEFRELSREVARRSIVLLKNDGILPLSKNIRTVAVIGPNADNPRNLHGDYSYTAHIPSVSETLEGVKIPEECAVRTVSILEGIKNKVSAETQVLYAKGCEILSDSKEGFDEAIEIAKRADVIIAVMGEESGLFHRGISGEGNDRTTLELFGIQRDLLRELHKLGKPIVLVLVNGRPQALKWEHENLNAILEAWYPGEEGGDAVADVIFGDYNPSGKLPISFPAVTGQVPVYYNRKPSAFTDYVEESAKPLYPFGHGLSYTTFEYSNLKIHPEKVNALEKVEISFTIKNTGVREGEEVVQLYVHDQVASLERPVKELKGFKKIHLKPGESKRVTFILYPEQLAFYDEFMRFVVEKGIFEIMIGSSSEDIRLTGTFEVLETKVITEKRKFASEIKVE

Xinyi Tong et al. (2021) — High-level expression of a novel multifunctional GH3 family β-xylosidase/α-arabinosidase/β-glucosidase from Dictyoglomus turgidum in Escherichia coli

Bioorganic Chemistry · doi:10.1016/j.bioorg.2021.104906 ↗ · Activity - Classical

24 measurements ▼

Database ID

UniProt: B8E050 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	25%	100	91	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	104	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15%	100	104	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100	106	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	25%	100	102	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	12	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	46	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	86	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	95	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	95	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	94	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	95	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	94	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100	19	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	40%	100	51	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100	98	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	5%	100	102	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	103	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	15%	100	102	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100	102	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	5%	100	101	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	50%	100	11	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	40%	100	81	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	30%	100	100	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	25%	100	91	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	104	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15%	100	104	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100	106	%	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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Structure

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