Penicillin V acylase from Streptomyces lavendulae

Enzyme Description

Species

Extremophile

EC Number

3.5.1.11 ↗

Sequence

Length: 806 amino acids

MTFRNRLRLFAVSGLALFTVSASLPPAAASGAPEARHPSGGGLSATVRYTEYGIPHIVAKDYANLGFGTGWAQAADQVCTLADGFVTVRGERSKFFGPDAAPDFSLSSAAKNLSSDLYFRGVRDSGTVEKLLKVPAPAGPSRDAKESMRGFAAGYNAWLRQNRDRITDPACRGASWVRPVTALDVAVRGFALAVLGGQGRGIDGITAAQPPTAAPPAAGVTPKEAAAAAQRLLSTQNADMGSNAVAFRGSTTANGRGLLLGNPHYPWDGGRRFWQSQQTIPGELNVAGGSLLGSTTVSIGHNADVAWSHTVATGVTLNLHQLTLDPADPTVYLVDGKPQRMTQRTVAVPVKGAAPVTRTQWWTRYGPVVTSLGAALPLPWTASTAYALNDPNAVNLRSADTSLGFSKARSTAGIERALHRSQGLPWVNTIAADRSGNSFFSQSQVLPRITDELAARCSTPLGQATYPSAGLAVLDGSTSACALGSDRDAVQPGIFGPGRMPTLKNAPYVENSNDSAWLTNADRPLTGYERVFGTTATQRSIRTRGAIEDVAAMAERGRLRVTDLERQQLANRAPTGDLVAADVAKWCAALPGGTAVGSSGTPVDVSAACPVLRRWDRSVDSDSRGALLFDRFWRKAAAVPAAELWKVPFDAADPVRTPRGLNTAAPGVGKALADTVTELKAAGIALNAPLGEHQFVVRNGKRIPVGGGTESLGIWNKIEPVWNPAAGGYTEVSAGSSYIQAVGWDNSRCPVARTLLTYSQSSNPNSPHYSDQTRLFSGERWVTSRFCEKDIARSPQLKVVRVHERR

Miguel Arroyo et al. (2000) — Prediction of penicillin V acylase stability in water-organic co-solvent monophasic systems as a function of solvent composition

Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00183-6 ↗ · Stability - C50 Stability - Half-life

52 measurements ▼

Database ID

UniProt: Q539C0 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Streptomyces lavendulae

Experimental Data (52 measurements)

52 measurements — page 2 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Triethylene Glycol	8%	231.0	630.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	10%	231.0	53.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetonitrile	10%	231.0	18.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	10%	231.0	80.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	231.0	79.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	10%	231.0	231.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Diethylene Glycol	10%	231.0	533.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Triethylene Glycol	10%	231.0	770.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	20%	231.0	10.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	20%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	231.0	30.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	20%	231.0	277.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Diethylene Glycol	20%	231.0	1155.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetonitrile	5%	231.0	52.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	3%	231.0	120.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetonitrile	3%	231.0	120.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Methanol	3%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	3%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	3%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	3%	231.0	182.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid , penicillin V acylase	—	—	Classical aqueous control (in hours)

Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Glycerol	—	—	7.5	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Ethylene Glycol	—	—	10.8	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Pyridine	—	—	0.86	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Triethylene Glycol	—	—	2.25	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Diethylene Glycol	—	—	3.7	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	1.2	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	1,4-Dioxane	—	—	1.3	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Acetone	—	—	3.4	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Isopropanol	—	—	3.3	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	1-Propanol	—	—	2.7	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Acetonitrile	—	—	3.5	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	3.2	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	—	—	3.5	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Ethanol	—	—	5.5	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - C50	Solvent concentration (in M) that halves enzyme activity, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in the presence of organic solvent	Methanol	—	—	4.9	% (v/v)	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Not applicable control (in % (v/v))
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	8%	231.0	91.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Triethylene Glycol	20%	231.0	990.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	8%	231.0	116.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	8%	231.0	248.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Diethylene Glycol	8%	231.0	385.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Triethylene Glycol	8%	231.0	630.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	10%	231.0	53.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetonitrile	10%	231.0	18.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	10%	231.0	80.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	231.0	79.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	10%	231.0	231.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Diethylene Glycol	10%	231.0	533.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Triethylene Glycol	10%	231.0	770.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	20%	231.0	10.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	20%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	231.0	30.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	20%	231.0	277.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Diethylene Glycol	20%	231.0	1155.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetonitrile	5%	231.0	52.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	3%	231.0	120.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetonitrile	3%	231.0	120.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Methanol	3%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	3%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	3%	231.0	178.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	3%	231.0	182.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Diethylene Glycol	3%	231.0	289.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Triethylene Glycol	3%	231.0	495.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	5%	231.0	90.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Methanol	8%	231.0	103.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Methanol	5%	231.0	151.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethylformamide (DMF)	5%	231.0	165.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	5%	231.0	169.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Ethylene Glycol	5%	231.0	239.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Diethylene Glycol	5%	231.0	365.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Triethylene Glycol	5%	231.0	533.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetone	8%	231.0	90.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)
Stability - Half-life	Half-life (in hours) in organic solvent, measured by absorbance spectrophotometry (colorimetric assay, 6-aminopenicillanic acid and p-dimethylaminobenzaldehyde reaction product absorbance measurement) in aqueous phase	Acetonitrile	8%	231.0	30.0	hours	100 mM phosphate buffer	8	40°C	22.5 mg/ml Penicillin V (Phenoxymethylpenicillin)	6-aminopenicillanic acid, penicillin V acylase	—	—	Classical aqueous control (in hours)

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Visualization : Stability — Half-life

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Visualization : Stability — C₅₀

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Structure

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