β-glucosidase from Alicyclobacillus herbarius

Enzyme Description

Species

Alicyclobacillus herbarius ↗

Extremophile

Yes "acidophilic" organism

EC Number

3.2.1.21 ↗

Sequence

Length: 452 amino acids

MTREFISFPQDFLFGTATASYQIEGAVHEDGRGESIWDRFSHTPGKVYQGHTGDVACDHYHRYREDVALMKELGIPAYRFSIAWPRIFPEKGMKNEAGLDFYRRLLEALHEADIRSFVTLYHWDLPQWLQDRGGWANRDTAEYFAEYASLIYERLGDGIDAFITHNEPWCAAFLGHGFGVHAPGHTDWREAFQAAHHILYSHGLAVQAHRASSHKGQIGITLNFTWVDAATDSATDQAAAEVSHAFNNRWFLEPVAGRGYPQEFQQLVEQRIGQFDFVRQGDLAVIAEPIDFLGINFYTRSVVAANPDDALFGLRTLEAPADNRTEMGWEIHPDSLYRLLTWVQSVTGQLPLYITENGAAFADEPVNGRVEDVRRIHYIADHLEAAKRFVDAGGPLKGYFLWSFMDNFEWALGYSKRFGMVYVDYESQQRLVKDSGRWFSEQIAAHKGQVRA

Lidia Delgado et al. (2020) — Release of Soybean Isoflavones by Using a β-Glucosidase from Alicyclobacillus herbarius

ChemBioChem · doi:10.1002/cbic.202000688 ↗ · Activity - Classical Stability - Incubation

96 measurements ▼

Database ID

UniProt: A0A8I3B065 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (96 measurements)

96 measurements — page 5 of 5

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	81	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	96	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	12	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	82	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	73	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	81	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	15	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	87	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	72	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	17	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	85	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	83	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	87	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	91	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	88	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	93	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	59	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	124	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	78	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	106	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetonitrile	20%	100	53	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetonitrile	10%	100	70	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	117	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	139	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	143	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	173	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	156	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	174	%	50 mM HEPES buffer	7.4	25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	81	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	66	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	81	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	88	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	93	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	87	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	73	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	14	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	70	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	69	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	78	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	83	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	83	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	65	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	12	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	65	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	62	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	66	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	71	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	75	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	1	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	69	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	16	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	22	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	44	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	46	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	53	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	49	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	15	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	75	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	68	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	61	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	17	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	79	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	58	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	19	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	87	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	84	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	83	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	90	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	97	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	82	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	78	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	96	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	84	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	97	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	99	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	108	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	88	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	13	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	74	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	67	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	76	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	17	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	85	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	80	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	18	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	104	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	95	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	109	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	102	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	101	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	107	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	80	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	78	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	71	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	79	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	75	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	81	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	96	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	12	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	82	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	73	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	81	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	15	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	87	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	72	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (5 days at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	17	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (48 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	85	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	83	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	87	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	91	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (1 hour at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	88	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	93	%	Incubation: ?, Assay: 50 mM HEPES buffer	Incubation: ?, Assay: 7.4	Incubation: 25°C, Assay: 25°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether assay and control were in organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation