Lipase (gene BTL2) from Bacillus thermocatenulatus

Enzyme Description

Species

Bacillus thermocatenulatus ↗

Extremophile

Yes thermophilic organism, "thermoalkalophilic" enzyme

EC Number

3.1.1.3 ↗

Sequence

Length: 388 amino acids

ASPRANDAPIVLLHGFTGWGREEMLGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLKAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSIPGAEKLNQWVQASPNTYYLSFSTERTHRGALTGNYYPELGMNAFSAVVCAPFLGSYRNEALGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTCNVDHLEVIGVDPNPSFDIRAFYLRLAEQLASLRP

Mohamed Shehata et al. (2020) — Understanding thermal and organic solvent stability of thermoalkalophilic lipases: insights from computational predictions and experiments

Journal of Molecular Modeling · doi:10.1007/s00894-020-04396-3 ↗ · Stability - Incubation

56 measurements ▼

Database ID

UniProt: Q59260 ↗

Sequence Annotation

Explicit - Provided PDB Accession Number (first 29 residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (56 measurements)

56 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	70%	100	100	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	30%	100	120	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	40%	100	122	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	50%	100	132	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	60%	100	173	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	70%	100	156	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	80%	100	9	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	90%	100	0	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	10%	100	78	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	20%	100	98	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	30%	100	133	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	40%	100	147	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	50%	100	173	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	60%	100	137	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	20%	100	112	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	10%	100	85	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	20%	100	142	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	30%	100	165	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	40%	100	179	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	50%	100	199	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone , Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions

Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	70%	100	100	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	30%	100	120	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	40%	100	122	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	50%	100	132	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	60%	100	173	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	70%	100	156	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	80%	100	9	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	90%	100	0	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	10%	100	78	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	20%	100	98	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	30%	100	133	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	40%	100	147	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	50%	100	173	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Toluene	60%	100	137	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	20%	100	112	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	10%	100	85	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	20%	100	142	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	30%	100	165	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	40%	100	179	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	50%	100	199	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	60%	100	240	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	70%	100	278	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	25%	100	107	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 60°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	25%	100	490	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 60°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	50%	100	245	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 60°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	50%	100	1	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 60°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Cyclohexane	70%	100	270	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 60°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	70%	100	0	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 60°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	60%	100	116	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	20%	100	162	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	30%	100	189	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	40%	100	230	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	50%	100	274	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	60%	100	325	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	70%	100	346	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	80%	100	287	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	90%	100	208	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	10%	100	114	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	20%	100	129	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	30%	100	149	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	40%	100	165	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	50%	100	138	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Ethanol	10%	100	149	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	70%	100	105	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	80%	100	30	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Methanol	90%	100	4	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	10%	100	213	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	20%	100	242	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	30%	100	266	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	40%	100	292	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	50%	100	353	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	60%	100	389	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	70%	100	428	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	80%	100	476	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Isopropanol	90%	100	472	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by fluorescence spectrophotometry (fluorescent product, measured with an excitation at 355 nm, and fluorescence emission measurement at 460 nm) in aqueous phase	Acetone	10%	100	104	%	Incubation: ?, Assay: 25 mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 25°C, Assay: 25°C	? mM 4-Methylumbelliferyl butyrate	4-Methylumbelliferone, Butyric Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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