Laccase 10 (gene LACC10) from Pleurotus ostreatus HAUCC 162

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 533 amino acids

MFPGARILATLTLALHLLHGTHAAIGPAGNMYIVNEDVSPDGFARSAVVARSVPATDPTPATASIPGVLVQGNKGDNFQLNVVNQLSDTTMLKTTSIHWHGFFQAGSSWADGPAFVTQCPVASGDSFLYNFNVPDQAGTFWYHSHLSTQYCDGLRGPFVVYDPSDPHLSLYDIDNADTVITLEDWYHIVAPQNAAIPTPDSTLINGKGRYAGGPTSPLAIINVESNKRYRFRLVSMSCDPNFTFSIDGHSLLVIEADAVNIVPITVDSIQIFAGQRYSFVLTANQAVDNYWIRANPNLGSTGFVGGINSAILRYAGATEDDPTTTSSTSTPLLETNLVPLENPGAPGPPVPGGADININLAMAFDFTTFELTINGVPFLPPTAPVLLQILSGASTAASLLPSGSIYELEANKVVEISMPALAVGGPHPFHLHGHTFDVIRSAGSTTYNFDTPARRDVVNTGTGANDNVTIRFVTDNPGPWFLHCHIDWHLEIGLAVVFAEDVTSISAPPAAWDDLCPIYNALSDNDKGGIVPS

Rui Zhuo et al. (2019) — The roles of Pleurotus ostreatus HAUCC 162 laccase isoenzymes in decolorization of synthetic dyes and the transformation pathways

Chemosphere · doi:10.1016/j.chemosphere.2019.06.113 ↗ · Activity - Classical Stability - Incubation

80 measurements ▼

Database ID

UniProt: A0A1S6YJF7 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Pichia pastoris GS115

Experimental Data (80 measurements)

80 measurements — page 1 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	20%	100.0	48.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	10%	100.0	72.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	20%	100.0	72.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	10%	100.0	92.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	20%	100.0	75.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	10%	100.0	84.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	20%	100.0	14.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	10%	100.0	36.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	20%	100.0	9.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	10%	100.0	31.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	20%	100.0	30.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	20%	100.0	21.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	10%	100.0	44.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	20%	100.0	22.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	10%	100.0	55.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	20%	100.0	7.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	10%	100.0	41.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	20%	100.0	30.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	10%	100.0	60.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	10%	100.0	64.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	20%	100.0	48.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	10%	100.0	72.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	20%	100.0	72.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	10%	100.0	92.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	20%	100.0	75.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	10%	100.0	84.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	20%	100.0	14.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	10%	100.0	36.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	20%	100.0	9.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	10%	100.0	31.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	20%	100.0	30.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	20%	100.0	21.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	10%	100.0	44.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	20%	100.0	22.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	10%	100.0	55.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	20%	100.0	7.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	10%	100.0	41.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	20%	100.0	30.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	10%	100.0	60.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	10%	100.0	64.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	44.62	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	58.03	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	46.73	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	53.9	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	62.61	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	94.19	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	62.0	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	93.81	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	64.25	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	92.62	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	57.95	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	84.84	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	70.62	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	54.54	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	77.99	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	58.77	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	21.27	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	57.28	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	40.78	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	61.0	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	50.81	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	59.74	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	51.22	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	65.82	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	47.53	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	67.32	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	45.21	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	68.48	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	54.06	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	71.04	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	94.57	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	80.57	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	81.76	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	98.31	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	88.89	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	96.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	35.3	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	94.46	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	82.67	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	94.6	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	81.03	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	94.36	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	87.47	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	95.89	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	94.1	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	65.35	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	95.42	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	98.56	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	91.16	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	99.59	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	99.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	65.66	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	64.06	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	62.88	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	76.89	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	63.95	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	26.37	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	70.57	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	61.89	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	73.33	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation