Laccase 9 (gene LACC9) from Pleurotus ostreatus HAUCC 162

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 529 amino acids

MFPGARILATLTLALHLLHGTHAAIGPTGDMYIVNEDVSPDGFTRSAVVARSDPTTNGTSETLTGVLVQGNKGDNFQLNVLNQLSDTTMLKTTSIHWHGFFQSGSTWADGPAFVNQCPIASGNSFLYDFNVPDQAGTFWYHSHLSTQYCDGLRGPFIVYDPSDPHLSLYDVDNADTIITLEDWYHVVAPQNAVLPTADSTLINGKGRFAGGPTSALAVINVESNKRYRFRLISMSCDPNFTFSIDGHSLQVIEADAVNIVPIVVDSIQIFAGQRYSFVLNANQTVDNYWIRADPNLGSTGFDGGINSAILRYAGATEDDPTTTSSTSTPLEETNLVPLENPGAPGPAVPGGADININLAMAFDVTNFELTINGSPFKAPTAPVLLQILSGATTAASLLPSGSIYSLEANKVVEISIPALAVGGPHPFHLHGHTFDVIRSAGSTTYNFDTPARRDVVNTGTDANDNVTIRFVTDNPGPWFLHCHIDWHLEIGLAVVFAEDVTSITAPPAAWDDLCPIYDALSDSDKGGIA

Rui Zhuo et al. (2019) — The roles of Pleurotus ostreatus HAUCC 162 laccase isoenzymes in decolorization of synthetic dyes and the transformation pathways

Chemosphere · doi:10.1016/j.chemosphere.2019.06.113 ↗ · Activity - Classical Stability - Incubation

80 measurements ▼

Database ID

UniProt: C6L8E2 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Pichia pastoris GS115

Experimental Data (80 measurements)

80 measurements — page 4 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	31.52	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	73.97	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	55.36	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	78.74	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	43.83	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	31.03	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	66.56	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	88.21	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	51.5	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	94.6	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	30.26	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	39.78	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	34.76	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	51.99	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	30.03	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	58.92	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	36.58	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	55.29	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	32.57	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	54.15	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	20%	100.0	24.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	10%	100.0	59.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	20%	100.0	26.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	10%	100.0	59.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	20%	100.0	49.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	10%	100.0	64.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	20%	100.0	6.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	10%	100.0	9.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	20%	100.0	2.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	10%	100.0	20.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	20%	100.0	8.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	20%	100.0	18.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	10%	100.0	49.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	20%	100.0	9.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	10%	100.0	36.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	20%	100.0	8.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	10%	100.0	25.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	20%	100.0	14.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	10%	100.0	47.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	10%	100.0	25.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	24.29	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	45.15	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	28.88	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	32.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	30.87	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	93.94	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	41.04	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	86.67	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	60.81	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	94.93	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	39.38	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	66.56	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	47.35	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	30.96	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	70.6	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	49.34	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	27.95	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	51.25	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	27.02	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	51.72	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	27.08	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	56.53	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	42.7	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	56.84	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	32.4	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	63.6	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	47.49	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	66.24	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	40.83	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	68.99	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	99.1	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	51.35	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	56.65	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	63.24	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	52.23	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	61.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	74.16	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	80.63	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	42.35	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	59.85	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	31.52	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	73.97	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	55.36	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	78.74	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	43.83	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	31.03	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	66.56	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	88.21	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	51.5	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	94.6	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	30.26	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	39.78	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	34.76	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	51.99	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	30.03	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	58.92	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	36.58	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	55.29	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	32.57	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	54.15	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation