Laccase (gene Lac1) from Trametes polyzona KU-RNW027

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 520 amino acids

MSRFNQLLAFVIASLSAVALAAVGPVADLTISNAEISPDGFPRQAVVVNGVSPGPIISGNKGDRFQLNVIDNLTNHTMLKSTSIHWHGFFQHGTNWADGPAFINQCPVSPGHAFLYDLQVPDQAGTFWYHSHLSTQYCDGLRGAFVVYDPNDPHKSRYDVDNDDTVITLSDWYHVAAKLGPRFPLGADATLINGKGRSPSTVSADLAVINVTAGKRYRFRLASLSCDPNHVFSIDGHTMTIIEADSVNTQPLEVDSIQIFAAQRYSFVLEANQPVDNYWIRANPNFGNTGFAGGINSAILRYDGAPAVEPTTTQTASTKPLNEVDLHPLESMPVPGKPVAGGVDKAINMAFNFNGTNFFINGASFVPPTVPVLLQILSGAQTAQDLLPEGSIYVLPNNADIEISFPATTAAPGAPHPFHLHGHTFAVVRSAGSTEYNYDNPIFRDVVSTGTPAANDNVTIRFRTDNPGPWFLHCHIDFHLEGGFAVVMAEDTPDVKAVNPVPQAWSDLCPTYDALDPNDQ

Piyangkun Lueangjaroenkit et al. (2019) — Two Manganese Peroxidases and a Laccase of Trametes polyzona KU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

Mycobiology · doi:10.1080/12298093.2019.1589900 ↗ · Stability - Incubation

30 measurements ▼

Database ID

UniProt: A0A2Z5WAB3 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, fungus Trametes polyzona KU-RNW027

Experimental Data (30 measurements)

30 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	10%	100.0	76.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	50%	100.0	13.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	40%	100.0	18.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	30%	100.0	32.5	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	20%	100.0	44.8	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	10%	100.0	71.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	50%	100.0	13.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	40%	100.0	29.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	30%	100.0	37.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	20%	100.0	52.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	10%	100.0	65.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	50%	100.0	6.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	40%	100.0	9.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	30%	100.0	17.5	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	20%	100.0	31.8	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	10%	100.0	99.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	50%	100.0	96.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	40%	100.0	98.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	30%	100.0	100.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	20%	100.0	100.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	10%	100.0	105.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	50%	100.0	9.7	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	40%	100.0	14.9	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	30%	100.0	24.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	20%	100.0	44.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	10%	100.0	68.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	50%	100.0	33.8	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	40%	100.0	39.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	30%	100.0	44.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	20%	100.0	60.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	10%	100.0	76.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	50%	100.0	13.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	40%	100.0	18.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	30%	100.0	32.5	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	20%	100.0	44.8	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	10%	100.0	71.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	50%	100.0	13.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	40%	100.0	29.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	30%	100.0	37.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	20%	100.0	52.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

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Visualization : Stability — Incubation

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