Manganese peroxidase (gene MnP1) from Trametes polyzona KU-RNW027

Enzyme Description

Species

Extremophile

EC Number

1.11.1.13 ↗

Sequence

Length: 364 amino acids

MAFKTLASFVSLLAAFQVANSAVTRRVTCSNGQQTANAACCALFPILDDIQQNLFDGGECGEEVHESLRLTFHDAIGISPKIAATGVFGGGGADGSIAIFEDIETNFHANNGVDEIIDEQKPFIARHNISTADFIQFAGAIGVSNCPGAPRLDVFIGRKDATQPAPDKTVPEPFDSVDSILARFADAGRFSPAEVVALLASHTVAAADHVDPSIPGTPFDSTPELFDTQFFIETQLKGTLFPGTAGNQGEVESPVRGELRLQSDFELARDQRTACEWQSFVNNQRKLQSAFKAAFRKMTVLGHNVRDLIDCSDVVPNPPAPASQAHFPAGLSHRDVEQACRQTPFPVLPTDPGPATSVAPVPPS

Piyangkun Lueangjaroenkit et al. (2019) — Two Manganese Peroxidases and a Laccase of Trametes polyzona KU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

Mycobiology · doi:10.1080/12298093.2019.1589900 ↗ · Stability - Incubation

30 measurements ▼

Database ID

UniProt: A0A2Z5WAB1 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, fungus Trametes polyzona KU-RNW027

Experimental Data (30 measurements)

30 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	10%	100.0	159.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	50%	100.0	37.9	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	40%	100.0	77.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	30%	100.0	132.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	20%	100.0	132.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	10%	100.0	127.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	50%	100.0	22.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	40%	100.0	32.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	30%	100.0	84.7	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	20%	100.0	112.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	10%	100.0	119.5	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	50%	100.0	74.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	40%	100.0	84.7	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	30%	100.0	97.9	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	20%	100.0	105.3	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Acetone	10%	100.0	130.5	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	50%	100.0	155.8	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	40%	100.0	153.2	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	30%	100.0	151.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	20%	100.0	151.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Butanol	10%	100.0	151.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	50%	100.0	16.3	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	40%	100.0	56.3	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	30%	100.0	85.3	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Isopropanol	20%	100.0	107.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	10%	100.0	126.8	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	50%	100.0	89.7	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	40%	100.0	100.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	30%	100.0	130.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	20%	100.0	141.0	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	1-Propanol	10%	100.0	159.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	50%	100.0	37.9	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	40%	100.0	77.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	30%	100.0	132.6	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	20%	100.0	132.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Ethanol	10%	100.0	127.4	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	50%	100.0	22.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	40%	100.0	32.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	30%	100.0	84.7	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation reaction products absorbance measurement, 469 nm) in aqueous phase	Methanol	20%	100.0	112.1	%	Incubation: ?, Assay: 50 mM malonate buffer, 0.2 MM H₂O₂ (Hydrogen Peroxide), 1mM MnSO₄	Incubation: ?, Assay: 4.5	Incubation: 4°C, Assay: 37°C	1 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxy radical	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

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Visualization : Stability — Incubation

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