GH3 β-glucosidase (gene MtBgl85) from Microbulbifer thermotolerans DAU221

Enzyme Description

Species

Extremophile

EC Number

3.2.1.21 ↗

Sequence

Length: 772 amino acids

MKKYPIYIAAMAGLLSLAATLPLQAANESVATTQSHSQQNAIESRIQSLLAQMSVQEKIGQLALRDWGTFKAEDMKDIKQAIREGRVGGFLNVNFSSVDNNAFEELQRLAVEESPRGIPLLFGQDVIHGYETIFPIPLGQAASWNPELIRNGARIAAQEASADGIRWTFAPMIDISRDPRWGRIAETLGEDPLLTSILGVAMVEGFQTDNLSDPTSLAACGKHFAGYGAAEGGRDYNSAYIPERLLRDIYLPPFKAGIDAGMQSIMSTYSSLNDVPGTGSPFLFKQILRDEWGFDGFVVSDWNAVMEMIPHGYARDAKHAAALAANAGIDMEMHTDTYEQFLARLMDEGEFTEAQLDTAVANILRVKLRLGLWENPYPRAKTPAEREQIIRNDQFLNAAKEAAKETFVLLKNDKQLLPLKKGQTVALIGPLADAPHEQMGTWVYNGNKEHSRTLLPALRAMVGDKGKILYAAGLEYSRDTSTRGFKAAIKAAKKADVVLFVGGEEAILSGEGHSRGDIRLPGAQAQLLAELAKTGKPLAMVLMAGRPLQLDQTLEQADAVMMAWHPGTMAGPALAEVLYGETSPSGRLPLSWPVGAGQIPIYYNHLPTGRPATDDNYTRIEKIGREVFQHQPGNSSNLLDYGHKPLFPFGYGLTYSEFHYSELELSDSTLDADGQLHVSATITNTGKRTATETVQLYVRDLVGSVSRPVRELKGFERITLAPGESRRVSFTLKANQLAFHNAQMQQVIEPGEFHVWIAPDAESGLQGSFTLQ

Hyo-Min Pyeon et al. (2019) — Cloning, purification, and characterization of GH3 Œ≤ -glucosidase, MtBgl85, from Microbulbifer thermotolerans DAU221

PeerJ · doi:10.7717/peerj.7106 ↗ · Activity - Classical

33 measurements ▼

Database ID

UniParc: UPI0008E19425 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (33 measurements)

33 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	20%	100	0	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	20%	100	16	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	29	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	10%	100	103	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	10%	100	87	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	10%	100	68	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isoamyl Alcohol	10%	100	97	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	10%	100	33	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	10%	100	78	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	10%	100	93	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	85	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	10%	100	20	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	100	124	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol , β-D-glucose	—	—	Correct contol (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	100	1	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	20%	100	48	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isoamyl Alcohol	20%	100	85	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	20%	100	72	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	20%	100	115	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	20%	100	99	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	0	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	30%	100	2	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	30%	100	0	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	20%	100	22	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	30%	100	0	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	30%	100	1	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	30%	100	99	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isoamyl Alcohol	30%	100	139	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	30%	100	116	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	30%	100	103	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	30%	100	88	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	79	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	20%	100	12	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	100	15	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	20%	100	0	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	20%	100	16	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	29	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	10%	100	103	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	10%	100	87	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	10%	100	68	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isoamyl Alcohol	10%	100	97	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	10%	100	33	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	10%	100	78	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	10%	100	93	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	85	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	10%	100	20	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	100	124	%	20 mM sodium phosphate buffer	7	50°C	1 mM p-Nitrophenyl β-D-glucopyranoside	p-Nitrophenol, β-D-glucose	—	—	Correct contol (in %)

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Visualization : Activity — Classical

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