Esterase (gene EstAG1) from Staphylococcus saprophyticus AG1

Enzyme Description

Species

Extremophile

EC Number

3.1.1.1 ↗

Sequence

Length: 300 amino acids

MKSRLTTKFVNKYLLPHRSIIFSSDEAFEQFLNDREKINEKKHKQPETLNVKSDLDKQMLGDIQVFRFRFRHSHERKLLYIHGGYNTLQPSAFHWRFMDKLSLSTLSEVVMPIYPKTPEFHIDDTYNAIFEMYNKLLTEVDSDAIVIMGDGTGAALALSFVQQLKEKQRPLPSKLYLFSPLLDAQLDNEGITESLDKKDVIVDKDGVQRILNVWSHNLPLDDARVSPIYGELTDLPPIYMFGGAYEIYLPDMQRFAHILADKSQEIHFYEYKRMIHAFPLLPIRESHKVVKQIVETLKDE

Alisa Gricajeva et al. (2019) — Atypical organic-solvent tolerant bacterial hormone sensitive lipase-like homologue EstAG1 from Staphylococcus saprophyticus AG1: Synthesis and characterization

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.02.110 ↗ · Activity - Classical Stability - Incubation

43 measurements ▼

Database ID

UniProt: A0A481SHK3 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (43 measurements)

43 measurements — page 2 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	5%	100.0	74.52	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	0.5%	100.0	119.24	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.5%	100.0	134.44	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	0.5%	100.0	99.47	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	0.5%	100.0	89.48	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	tert-Butanol	0.5%	100.0	71.32	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	0.5%	100.0	37.2	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	0.5%	100.0	65.99	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	1%	100.0	127.09	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	1%	100.0	33.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100.0	147.63	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	1%	100.0	79.26	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	1%	100.0	45.25	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	tert-Butanol	1%	100.0	69.49	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	1%	100.0	32.6	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	1%	100.0	59.6	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	5%	100.0	92.45	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	5%	100.0	4.67	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	128.3	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	5%	100.0	52.62	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Correct contol (in %)

Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	151.49	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	tert-Butanol	5%	100.0	32.33	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	5%	100.0	61.82	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	5%	100.0	0.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	80.46	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100.0	0.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	143.82	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	10%	100.0	55.33	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	10%	100.0	63.93	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	tert-Butanol	10%	100.0	2.58	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	0.5%	100.0	22.07	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	0.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100.0	66.06	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100.0	0.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	139.17	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	20%	100.0	72.41	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	20%	100.0	0.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	tert-Butanol	20%	100.0	0.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	100.0	167.99	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100.0	0.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	5%	100.0	74.52	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	0.5%	100.0	119.24	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.5%	100.0	134.44	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	0.5%	100.0	99.47	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	0.5%	100.0	89.48	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	tert-Butanol	0.5%	100.0	71.32	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	0.5%	100.0	37.2	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	0.5%	100.0	65.99	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	1%	100.0	127.09	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	1%	100.0	33.0	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100.0	147.63	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1,4-Dioxane	1%	100.0	79.26	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	1%	100.0	45.25	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	tert-Butanol	1%	100.0	69.49	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	1%	100.0	32.6	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	1%	100.0	59.6	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	5%	100.0	92.45	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	5%	100.0	4.67	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	128.3	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	5%	100.0	52.62	%	50 mM Briton-Robinson buffer	7.5	45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Correct contol (in %)
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100.0	99.68	%	Incubation: ?, Assay: 50 mM Briton-Robinson buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about incubation temperature
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	163.42	%	Incubation: ?, Assay: 50 mM Briton-Robinson buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about incubation temperature
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1,4-Dioxane	50%	100.0	124.18	%	Incubation: ?, Assay: 50 mM Briton-Robinson buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about incubation temperature

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation