Alcohol dehydrogenase (gene adh/a1) from an uncharacterized MSBL1 archaeon collected

Enzyme Description

Species
Na (uncharacterized MSBL1 archaeon collected from the Atlantis II Deep interphase)
Extremophile
Yes likely extremophilic organism as it is from extreme environment (deep sea brine pool), "thermophilic" and "halophilic" enzyme
EC Number
1.1.1.1 β†—

Sequence

Length: 332 amino acids
MKAMQLKEPKPVEQAPLEMVELKEPRPGPKEVKIDVQACGVCHTDLHTVEGELSLPKLPIVPGHEVVGVVEESGDEAEHFEVGDRVGVTWLYSSCGECKFCRRGQENLCEDPMFTGLHADGGYEESMVAKEDFVYPIPKNISDEDAAPLLCAGVIGYRSLRLSEVKPGQRLGLFGFGASAHIVIQLATDMGCEVYVFTRSEEHRRLARELGSAWEGSAKDDPPHRIDSGITFAPVGWIVKEALRDLEKGGTLAINAIHMTPIPELDYDLIYHEKKLRSVANVTREDAEGFLKIAGDIPVQTEVETFPLEEANRALRLLKDSKINGAGVLKVS
Anastassja L. Akal et al. (2018) β€” A polyextremophilic alcohol dehydrogenase from the Atlantis II Deep Red Sea brine pool
FEBS Open Bio  Β· doi:10.1002/2211-5463.12557 β†—  Β· Stability - Incubation
47 measurements ▼
Database ID
UniProt: A0A133V8G4 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Haloferax volcanii H1895

Experimental Data (47 measurements)

47 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (8 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 73 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 68 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 62 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 62 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 56 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (8 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 53 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (16 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 33 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 32 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (32 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 30% 100 32 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 68 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 74 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 77 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (32 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 10% 100 66 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (16 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 70 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 61 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (32 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 70 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100 64 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100 54 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100 49 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100 53 % Incubation: 10 mM HEPES/NaOH, 2M NaCl, Assay: 50 mM glycine-NaOH buffer, 3 M KCl Incubation: 7.5, Assay: 10 Incubation: Room temperature, Assay: 60Β°C 0.2% (v/v) Cinnamyl alcohol Cinnamaldehyde 10 mM NADP+ β€” Water-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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