NAD(P) dependent formate dehydrogenase (gene fdh) from Burkholderia dolosa PC543

Enzyme Description

Species

Burkholderia dolosa PC543 ↗

Extremophile

No but organic "solvent-tolerant" and "acidic-tolerant" enzyme

EC Number

1.2.1.2 ↗

Sequence

Length: 386 amino acids

MATVLCVLYPDPVDGYPPHYVRDTIPVITRYADGQTAPTPAGPPGFRPGELVGSVSGALGLRGYLEAHGHTLIVTSDKDGPDSEFERRLPDADVVISQPFWPAYLTAERIARAPKLRLALTAGIGSDHVDLDAAARAHITVAEVTGSNSISVAEHVVMTTLALVRNYLPSHAIAQQGGWNIADCVSRSYDVEGMHFGTVGAGRIGLAVLRRLKPFGLHLHYTQRHRLDAAIEQELGLTYHADPASLAAAVDIVNLQIPLYPSTEHLFDAAMIARMKRGAYLINTARAKLVDRDAVVRAVTSGHLAGYGGDVWFPQPAPADHPWRAMPFNGMTPHISGTSLSAQARYAAGTLEILQCWFDGRPIRNEYLIVDGGTLAGTGAQSYRLT

Saadet Alpdağtaş et al. (2018) — DMSO tolerant NAD(P)H recycler enzyme from a pathogenic bacterium, Burkholderia dolosa PC543: effect of N-/C-terminal His Tag extension on protein solubility and activity

Engineering in Life Sciences · doi:10.1002/elsc.201800036 ↗ · Activity - Classical

30 measurements ▼

Database ID

UniParc: UPI00017992AD ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (30 measurements)

30 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	100	86	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	142	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100	107	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100	80	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	129	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	117	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	132	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	5%	100	116	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	5%	100	101	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	5%	100	126	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2 , H+	1 mM NADP+	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	30%	100	64	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	41	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	50%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	50%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	50%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	50%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	119	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	40%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	40%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	40%	100	32	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	40%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	172	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	30%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	30%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	30%	100	51	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	5%	100	130	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	158	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	20%	100	54	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	20%	100	0	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	20%	100	91	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	100	86	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	142	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100	107	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100	80	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	129	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	117	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	132	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	5%	100	116	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	5%	100	101	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	5%	100	126	%	64 mM sodium phosphate buffer	6.2	37°C	170 mM Sodium formate	CO2, H+	1 mM NADP+	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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Structure

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