Esterase (gene Est903) from a paper mill sludge metagenomic library

Enzyme Description

Species

Na (paper mill sludge metagenomic library)

Extremophile

No but "alkali-tolerant" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 300 amino acids

MVVGYSRVKDTSMRSVLCSWWFTFSAGIFAIPFSTGEDLLAIDSPAVKADVVYGHKDGMALTMDVFEPTGEANGAGLMFMVSGGWVSTWSPPDRMLPLIQPLLQRGYKVIAVRHGSSPRYVIPEIVSDVRLAHEFVHDHAKEWNIDPEKIGVFGFSAGGHLSLVLGTTSNGDADRKAGRKARIAAVAAVFPPTDLRPYMALDNPLREKFPALKFPLDQGPDYSPLLQVSPDDAPTVLIHGDKDELVPIWHSEKISSAFDELKVPTELLVIEGAAHGFDAEGNKRMFTAMVQWFDKHLLGK

Mei-Lu Jia et al. (2019) — Expression and characterization of an esterase belonging to a new family via isolation from a metagenomic library of paper mill sludge

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.01.025 ↗ · Stability - Incubation

28 measurements ▼

Database ID

UniParc: UPI000E104C98 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (28 measurements)

28 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	15%	100.0	122.6	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100.0	92.0	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	15%	100.0	103.1	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100.0	71.7	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	15%	100.0	89.1	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100.0	24.0	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	15%	100.0	70.5	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	119.1	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	15%	100.0	102.8	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	30%	100.0	40.7	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	15%	100.0	61.0	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100.0	31.3	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	15%	100.0	72.6	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100.0	78.5	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	15%	100.0	105.4	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100.0	34.5	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	15%	100.0	85.5	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100.0	42.9	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	15%	100.0	80.2	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100.0	9.6	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	15%	100.0	26.4	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	68.0	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	15%	100.0	125.0	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	30%	100.0	79.4	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	15%	100.0	80.2	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100.0	59.7	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	15%	100.0	91.7	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100.0	132.2	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	15%	100.0	122.6	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100.0	92.0	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	15%	100.0	103.1	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100.0	71.7	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	15%	100.0	89.1	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100.0	24.0	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	15%	100.0	70.5	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	119.1	%	Incubation: ?, Assay: 40 mM Britton-Robinson buffer	Incubation: ?, Assay: 9	Incubation: 4°C, Assay: 51°C	1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

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