Lipase (gene LipA9) from Marinobacter lipolyticus 27-A9

Enzyme Description

Species

Marinobacter lipolyticus 27-A9 ↗

Extremophile

Yes likely psychrophilic or psychrotrophic organism (from Antarctic ocean)

EC Number

3.1.1.3 ↗

Sequence

Length: 404 amino acids

MTESKQVTGLSSGHLTHIEDHLDRRYIQPGKLPGALTLVARRGEIAYLKAQGLMDVERNKPVCRDTVFRIYSMTKPITSIAMMQLYEQGRFLLDDPVHKYIPAWKNLRVYNSGVYPNFLTTPATSTMTIRDLFTHMSGLTYGFMNRTNVDAAYRELKLDGSRNLTLEALVGHLAELPLEFSPGTAWNYSVSTDVLGYLVQLLADQPFDEYLREHIFEPLAMSDTGFHVRDDQLDRFAACYQYDPVDQFKLQDDPQTSPFRDKRRFLSGGGGLVSTIDDYFHFAQALCQGGEFGGRRIIGRKTLEFMRRNHLPGNQDLPGLSVGPFSETPYAGTGFGLGFSVKTDVAKSQINGSVGEYGWGGLASTNFIIDPVEELVVIFMTQLIPSSTYPIRQELRAIVNGALV

Se Hyeon Park et al. (2018) — Characterization of Organic Solvent-Tolerant Lipolytic Enzyme from Marinobacter lipolyticus Isolated from the Antarctic Ocean

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-018-2865-5 ↗ · Stability - Incubation

54 measurements ▼

Database ID

UniParc: UPI000EF332DA ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	80%	100.0	47.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	60%	100.0	81.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	65%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	70%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	80%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	90%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	95%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100.0	104.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	20%	100.0	100.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100.0	102.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	40%	100.0	98.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100.0	97.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	60%	100.0	103.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	70%	100.0	98.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100.0	92.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	90%	100.0	28.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	95%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100.0	105.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	20%	100.0	100.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100.0	97.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control

Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	80%	100.0	47.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	60%	100.0	81.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	65%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	70%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	80%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	90%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	95%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100.0	104.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	20%	100.0	100.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100.0	102.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	40%	100.0	98.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100.0	97.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	60%	100.0	103.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	70%	100.0	98.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100.0	92.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	90%	100.0	28.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	95%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100.0	105.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	20%	100.0	100.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100.0	97.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	40%	100.0	102.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100.0	92.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	60%	100.0	94.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	70%	100.0	95.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	80%	100.0	73.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	90%	100.0	46.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	95%	100.0	41.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100.0	97.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100.0	96.8	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100.0	70.5	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	30%	100.0	93.2	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100.0	100.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1,2-Dimethoxyethane	30%	100.0	94.7	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100.0	100.5	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Propionitrile	30%	100.0	104.2	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	2-Butanone	30%	100.0	93.2	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	30%	100.0	113.2	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Diethyl Ether	30%	100.0	115.3	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	103.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	100.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	102.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	104.2	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	96.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	55%	100.0	70.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100.0	2.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	65%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	70%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	80%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	90%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	95%	100.0	0.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100.0	104.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	20%	100.0	105.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100.0	100.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	40%	100.0	101.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 8	Incubation: 25°C, Assay: 37°C	0.1 mM p-Nitrophenyl hexanoate	caproic acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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