Esterase (gene estUT1) from Ureibacillus thermosphaericus UT1
Enzyme Description
Extremophile
Yes
"thermophilic" organism
EC Number
Sequence
MKIVSPKPFTIETGKRAVLLLHGFTGNTNDVKRLGRYLADRNYTVHAPLYKGHGSDPLTLINTDPIEWWNSAVEGYDELRRRGYKEIAVAGVSLGGIFSLRLGEERPIKAIVTMSAPALSKSVDSLQQRIISYTINYKKLTGTYNEDVDKPENLAKTYRMPKLEYLQGIINDTSAKLNQIDKPVHILRGLNDDEYYCQSADYIYNSVKSRIKSVKSFINSGHILTVDKERDLVFEEVYRFFESLKWEE
Yuliya V. Samoylova et al. (2018)
β Cloning, expression and characterization of the esterase estUT1 from Ureibacillus thermosphaericus which belongs to a new lipase family XVIII
Extremophiles
Β· doi:10.1007/s00792-018-0996-9 β
Β· Stability - Incubation
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Database ID
UniProt: A0A223PZA8 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (36 measurements)
36 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 10% | 100 | 123 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 30% | 100 | 142 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | n-Hexane | 10% | 100 | 117 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | n-Hexane | 30% | 100 | 107 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 10% | 100 | 123 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 30% | 100 | 117 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 87 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 10% | 100 | 116 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Chloroform | 10% | 100 | 61 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Chloroform | 30% | 100 | 42 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 10% | 100 | 97 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 30% | 100 | 78 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | 1-Butanol | 10% | 100 | 96 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | 1-Butanol | 30% | 100 | 78 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 10% | 100 | 99 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 30% | 100 | 90 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 1% acetonitrile, 4% Ethanol | Incubation: 8, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.