Lipase (UniParc : UPI00021D549A) from a hot spring soil sample metagenomic library

Enzyme Description

Species

Na (hot spring soil sample metagenomic library)

Extremophile

No but "thermostable" enzyme

EC Number

3.1.1.3 ↗

Sequence

Length: 388 amino acids

ASPRANDAPIVLLHGFTGWGREEMLGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAVHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSQEEREYAKEHNVSLSPLFEGGHRFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLEAAAVASNVPYTSQVYDFKLDQWGLRRQPDESFDHYFERLKRSPVWTSTDTARYDLSVPGAETLNRWVKASPNTYYLSFSTERTYRGALTGNYYPELGMNAFSAIVCAPFLGSYRNAALGIDSHWLENDGIVNTISMNGPKRGSNDRIVPYDGTLKKGVWNDMGTYNVDHLEIIGVDPNPSFDIRAFYLRLAEQLASLRP

Nisha Chopra et al. (2018) — Structural and functional insights into thermostable and organic solvent stable variant Pro247-Ser of Bacillus lipase

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.10.176 ↗ · Stability - Incubation

48 measurements ▼

Database ID

UniParc: UPI00021D549A ↗

Sequence Annotation

Explicit (29 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements — page 2 of 3

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	99.8	—	71.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	99.8	—	54.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	99.8	—	13.5	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	99.8	—	33.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	100.1	17.8	52.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.1	13.5	79.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.1	30.7	73.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.1	42.0	75.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.1	28.1	62.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.1	12.3	62.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.1	36.9	70.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.1	15.5	60.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.1	46.6	84.1	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.1	38.2	85.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.1	28.1	75.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	100.1	23.9	77.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.1	47.0	80.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.1	53.2	79.3	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.1	51.7	84.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.1	41.5	88.1	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

WT	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	99.8	—	41.5	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	99.8	—	36.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	99.8	—	46.6	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	99.8	—	38.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	99.8	—	42.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	99.8	—	28.1	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	99.8	—	23.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	99.8	—	17.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	99.8	—	47.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	99.8	—	53.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	99.8	—	12.3	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	99.8	—	51.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	99.8	—	30.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	99.8	—	44.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	99.8	—	28.1	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	99.8	—	58.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	99.8	—	59.3	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	99.8	—	15.5	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	99.8	—	69.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	99.8	—	78.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	99.8	—	71.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	99.8	—	54.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	99.8	—	13.5	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
WT	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	99.8	—	33.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 50°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	100.1	17.8	52.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.1	13.5	79.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.1	30.7	73.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.1	42.0	75.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.1	28.1	62.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.1	12.3	62.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.1	36.9	70.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.1	15.5	60.9	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.1	46.6	84.1	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.1	38.2	85.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.1	28.1	75.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	100.1	23.9	77.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.1	47.0	80.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.1	53.2	79.3	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.1	51.7	84.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.1	41.5	88.1	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.1	44.4	81.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	100.1	33.4	87.0	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.1	54.7	89.1	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.1	71.0	93.4	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.1	78.8	91.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.1	69.9	94.8	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.1	59.3	90.2	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
P247S	Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	n-Hexane	50%	100.1	58.9	92.7	%	Incubation: ?, Assay: 40 mM sodium phosphate buffer, 0.08% sodium-deoxicholate, 10% Ethanol	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 45°C	0.1 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

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Mutations in this dataset (2)

WT P247S

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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