Vimelysin from Vibrio sp. T-1800

Enzyme Description

Species

Extremophile

EC Number

3.4.24.- ↗

Sequence

Length: 412 amino acids

AKSSGTGPGGNLKTTRYEYGSDFPSFSIDKTGTTCKLENDSVKTVNLNHGTSGSAAYSYNCADGTNYTDHKYINGAYSPLNDAHYFGNVVFDMYKEWMNTSPLTFQLTMRVHYSSNYENAFWNGSSMTFGDGGSTFYPLVDINVSAHEVSHGFTEQNSGLVYQNMSGGINEAFSDIAGEAAEYYLRGNVDWVVGSDIFKSEGGLRYFDQPSKDGRSIDHASQYYDGLNVHLSSGVYNRAFYLLANKSGWDVRKGFEIFTVANQLYWTANSTFDAGACGVAKAAADMGYVVADVEDAFNTVGVNGSCGSTPPTGNVLTKGTPIANLSGNQSSESFYTFTVDSASSATVSMSGGSGDADLYVKSGSKPTTSSYDCRPYRAGNNEQCSVSAQPGITYHVLLRGYSNYSGLTLRLD

Kohei Oda et al. (1996) — A Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800: Purification and Characterization

Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.60.463 ↗ · Activity - Classical

22 measurements ▼

Database ID

UniProt: Q76LC2 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Vibrio sp. T-1800

Experimental Data (22 measurements)

22 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	14	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	10	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	26	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	39	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	28	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	46	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	70	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	88	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	4	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	10%	100	61	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	25	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	2	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	16	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	21	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	35	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	30%	100	11	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	20%	100	18	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	10%	100	42	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)

—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	14	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	10	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	26	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	39	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	28	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	46	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	70	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	88	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	4	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	10%	100	61	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	25	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	2	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	16	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	21	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	35	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	30%	100	11	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	20%	100	18	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	10%	100	42	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	30%	100	16	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	20%	100	45	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

Kohei Oda et al. (1996)

One bar per measurement. Colour = solvent, shade = solvent volume.

Toshihiro Takahashi et al. (2005) — Molecular Cloning of the Gene Encoding Vibrio Metalloproteinase Vimelysin and Isolation of a Mutant with High Stability in Organic Solvents

The Journal of Biochemistry · doi:10.1093/jb/mvi173 ↗ · Stability - Incubation

8 measurements ▼

Database ID

UniProt: Q76LC2 ↗

Sequence Annotation

Explicit - Provided (first 195 residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli JM109

Experimental Data (8 measurements)

8 measurements

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	98	—	58	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	91	—	22	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	98	—	72	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	91	—	38	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	102	58	79	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	92	22	56	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	102	72	82	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	92	38	66	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2 , MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase

WT	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	98	—	58	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	91	—	22	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	98	—	72	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	91	—	38	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	102	58	79	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Ethanol	50%	92	22	56	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (1 hour at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	102	72	82	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase
N123D	Stability - Incubation	Activity after incubation (3 hours at 37°C), measured by fluorescence spectrophotometry (fluorophore MOCAc fluorescence measurement as it is separated from quencher DNP, excitation at 360 nm and absorbance measurement at 450 nm) in aqueous phase	Isopropanol	50%	92	38	66	%	Incubation: 50 mM Tris-Hcl, 5mM CaCl2, Assay: 50 mM MES buffer, 10 mM CaCl2 and 0.005% Triton X-100	Incubation: 7.5, Assay: 6.5	Incubation: 37°C, Assay: 25°C	MOCAc-PLGL-A2pr(Dnp)-AR-NH2	L-A2pr(Dnp)-AR-NH2, MOCAc-PLG	—	—	Non-incubated control (in %), assay in aqueous phase

Mutations in this dataset (2) — Toshihiro Takahashi et al. (2005)

WT N123D

Visualization : Stability — Incubation

Toshihiro Takahashi et al. (2005)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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