Esterase (gene EstDZ2) from a Kamtchatka hot spring metagenomic library

Enzyme Description

Species

Na (Kamtchatka hot spring metagenomic library)

Extremophile

Yes "thermophilic" organism

EC Number

3.1.1.1 ↗

Sequence

Length: 271 amino acids

MRTILYATLAVTLMSVSLSSAQPAPPFSFQVYTDVVYYDGPDAHPTKHRLDLFVPDGLQDAPVLIFVHGGGWTSGDKNLYAFIGQAFAAQGFATAVINYRLSPQVQHPAHIEDVARAFSWVYKNIARYGGNPEKIFVTGHSAGGHLTALLALDEKYLQAHGLTLAAIKGAIPISGVYDVTPVLALYRTVFGADPDQRWDASPLAHVGPNKPPFLILYAQFDFPGLGLQAQQLFAELRTHNNEATLTEIPNRDHVTIVATIGASNDPTTEEI

Dimitra Zarafeta et al. (2016) — Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases

Scientific Reports · doi:10.1038/srep38886 ↗ · Activity - Classical Stability - Incubation

48 measurements ▼

Database ID

UniProt: A0A1L2DXZ2 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements — page 3 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	50%	100	89	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isooctane	50%	100	90	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	50%	100	79	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	50%	100	89	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	50%	100	83	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	50%	100	95	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100	95	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	50%	100	95	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	30%	100	105	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	100	110	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	50%	100	0	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	30%	100	25	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	10%	100	40	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isooctane	50%	100	0	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isooctane	30%	100	25	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isooctane	10%	100	52	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	50%	100	0	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	30%	100	22	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	10%	100	113	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	50%	100	0	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	30%	100	2	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	50%	100	0	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	10%	100	18	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	50%	100	23	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	109	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	100	93	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	50%	100	6	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	10%	100	117	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	30%	100	67	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	50%	100	6	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	10%	100	88	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	30%	100	26	%	25 mM Tris-HCl, 0.05% Triton X-100	8	50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "reaction under constant agitation"	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	50%	100	92	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	50%	100	76	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isooctane	50%	100	89	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	50%	100	91	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	50%	100	94	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100	97	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	50%	100	92	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	50%	100	95	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	50%	100	100	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	50%	100	82	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isooctane	50%	100	92	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	50%	100	94	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	50%	100	90	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	50%	100	92	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100	98	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	50%	100	96	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	50%	100	89	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isooctane	50%	100	90	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	50%	100	79	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	50%	100	89	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	50%	100	83	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	50%	100	95	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100	95	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	50%	100	95	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 50°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation