Catalase from Bos taurus (bovine)
Enzyme Description
Sequence
MADNRDPASDQMKHWKEQRAAQKPDVLTTGGGNPVGDKLNSLTVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITRYSKAKVFEHIGKRTPIAVRFSTVAGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDALLFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNANGEAVYCKFHYKTDQGIKNLSVEDAARLAHEDPDYGLRDLFNAIATGNYPSWTLYIQVMTFSEAEIFPFNPFDLTKVWPHGDYPLIPVGKLVLNRNPVNYFAEVEQLAFDPSNMPPGIEPSPDKMLQGRLFAYPDTHRHRLGPNYLQIPVNCPYRARVANYQRDGPMCMMDNQGGAPNYYPNSFSAPEHQPSALEHRTHFSGDVQRFNSANDDNVTQVRTFYLKVLNEEQRKRLCENIAGHLKDAQLFIQKKAVKNFSDVHPEYGSRIQALLDKYNEEKPKNAVHTYVQHGSHLSAREKANL
Edmond Magner et al. (1995)
β The oxidation of chiral alcohols catalyzed by catalase in organic solvents
Biotechnology and Bioengineering
Β· doi:10.1002/bit.260460211 β
Β· Activity - Classical
▼
Database ID
UniProt: P00432 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (1 measurement)
1 measurement
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity (in nmol/(min*mg) measured by HPLC in the presence of organic solvent | Ethanol | 98% | 1 | 450 | nmol/(min*mg) | 100 mM phosphate buffer | 7 | 30Β°C | tert-Butyl hydroperoxide | H2O , tert-butanol | β | β | Correc control (in nmol/(min*mg)) |
Visualization : Activity β Classical
Edmond Magner et al. (1995)
One bar per measurement. Colour = solvent, shade = solvent volume.
Mohd. Rehan et al. (2006)
β Effect of organic solvents on the conformation and interaction of catalase and anticatalase antibodies
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2006.03.023 β
Β· Activity - Classical
▼
Database ID
UniProt: P00432 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (14 measurements)
14 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 2% | 100 | 96 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 8% | 100 | 94 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 89 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 84 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40% | 100 | 79 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 44 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 60% | 100 | 8 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 5% | 100 | 138 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100 | 132 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100 | 131 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100 | 120 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 40% | 100 | 98 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 100 | 100 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (H2O2 absorbance measurement, 570 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 60% | 100 | 94 | % | 20 mM sodium phosphate buffer | 7 | 30Β°C | HβOβ (Hydrogen Peroxide) | H2O , Oβ | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
Mohd. Rehan et al. (2006)
One bar per measurement. Colour = solvent, shade = solvent volume.
Adina Raducan et al. (2012)
β Kinetics of hydrogen peroxide decomposition by catalase: hydroxylic solvent effects
Bioprocess and Biosystems Engineering
Β· doi:10.1007/s00449-012-0742-0 β
Β· Activity - Classical
▼
Database ID
UniProt: P00432 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (49 measurements)
49 measurements β page 2 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Glycerol | 0.038 (molar ratio of nonaqueous component)% | 7.28 | 5.39 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Glycerol | 0.049 (molar ratio of nonaqueous component)% | 7.28 | 5.57 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Glycerol | 0.057 (molar ratio of nonaqueous component)% | 7.28 | 5.49 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Glycerol | 0.074 (molar ratio of nonaqueous component)% | 7.28 | 5.69 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Glycerol | 0.0123 (molar ratio of nonaqueous component)% | 7.28 | 5.54 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Ethanol | 0.031 (molar ratio of nonaqueous component)% | 7.28 | 4.12 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.03 (molar ratio of nonaqueous component)% | 7.28 | 4.01 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.044 (molar ratio of nonaqueous component)% | 7.28 | 3.06 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.059 (molar ratio of nonaqueous component)% | 7.28 | 2.68 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.074 (molar ratio of nonaqueous component)% | 7.28 | 2.2 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.089 (molar ratio of nonaqueous component)% | 7.28 | 2.08 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.104 (molar ratio of nonaqueous component)% | 7.28 | 1.7 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.119 (molar ratio of nonaqueous component)% | 7.28 | 1.31 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.133 (molar ratio of nonaqueous component)% | 7.28 | 0.85 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.147 (molar ratio of nonaqueous component)% | 7.28 | 0.52 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Ethanol | 0.01 (molar ratio of nonaqueous component)% | 7.28 | 7.07 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Ethanol | 0.02 (molar ratio of nonaqueous component)% | 7.28 | 4.86 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Methanol | 0.018 (molar ratio of nonaqueous component)% | 7.28 | 4.47 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Ethanol | 0.041 (molar ratio of nonaqueous component)% | 7.28 | 3.39 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
| Activity - Classical | Initial reaction rate (in 100 000* M*s^-1), measured by absorbance spectrophotometry (decrease in H2O2 absorbance measurement, 240 nm) | Ethanol | 0.046 (molar ratio of nonaqueous component)% | 7.28 | 2.33 | 100 000 * M * s^-1 | Distilled water | About 7 | 25Β°C | 0.02 M HβOβ (Hydrogen Peroxide) | H2O + O2 | β | β | Classical aqueous control (in 100 000 * M * s^-1) |
Visualization : Activity β Classical
Adina Raducan et al. (2012)
One bar per measurement. Colour = solvent, shade = solvent volume.