X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis

Enzyme Description

Species

Extremophile

EC Number

3.4.14.5 ↗

Sequence

Length: 763 amino acids

MRFNHFSIVDKNFDEQLAELDQLGFRWSVFWDEKKILKDFLIQSPSDMTALQATAELDVIEFLKSSIELDWEIFWNIALQLLDFVPNFDFEIGKAFEYAKNSNLPQIEAEMTTENIISAFYYLLCTRRKTGMILVEHWVSEGLLPLDNHYHFFNDKSLATFDSSLLEREVLWVESPVDSEQRGENDLIKIQIIRPKSTEKLPVVMTASPYHLGINDKANDLALHDMNVELEEKTSHEIHVEQKLPQKLSAKAKELPIVDKAPYRFTHGWTYSLNDYFLTRGFASIYVAGVGTRSSDGFQTSGDYQQIYSMTAVIDWLNGRARAYTSRKKTHEIKASWANGKVAMTGKSYLGTMAYGAATTGVEGLELILAEAGISSWYNYYRENGLVRSPGGFPGEDLDVLAALTYSRNLDGADFLKGNAEYEKRLAEMTAALDRKSGDYNQFWHDRNYLINTDKVKADVLIVHGLQDWNVTPEQAYNFWKALPEGHAKHAFLHRGAHIYMNSWQSIDFSETINAYFVAKLLDRDLNLNLPPVILQENSKDQVWTMMNDFGANTQIKLPLGKTAVSFAQFDNNYDDETFKKYSKDFNVFKKDLFENKANEAVIDLELPSMLTINGPVELELRLKLNDTKGFLSAQILDFGQKKRLEDKVRVKDFKVLDRGRNFMLDDLVELPLVESPYQLVTKGFTNLQNQSLLTVSDLKADEWFTIKFELQPTIYHLEKADKLRVILYSTDFEHTVRDNRKVTYEIDLSQSKLIIPIESVKN

J.F. Chich et al. (1995) — Preparation of Bacterial X-Prolyl Dipeptidyl Aminopeptidase and Its Stabilization by Organic Cosolvents

Analytical Biochemistry · doi:10.1006/abio.1995.1036 ↗ · Stability - Incubation

80 measurements ▼

Database ID

UniProt: P22346 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Lactococcus lactis

Experimental Data (80 measurements)

80 measurements — page 4 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	75%	100	29	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	75%	100	11	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	28	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	121	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	90%	100	11	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	75%	100	58	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100	104	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	25%	100	103	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	90%	100	13	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	25%	100	123	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100	42	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	25%	100	100	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	75%	100	12	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100	67	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	25%	100	128	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	75%	100	12	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	34	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")

Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	75%	100	9	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	90%	100	11	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	75%	100	17	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	50%	100	92	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	25%	100	116	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	75%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	8	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	78	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	90%	100	9	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	25%	100	68	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100	9	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	25%	100	56	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	90%	100	0	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	75%	100	0	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100	4	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	25%	100	52	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	90%	100	0	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	75%	100	0	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100	4	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	75%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	90%	100	6	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	75%	100	8	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	50%	100	8	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	25%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	90%	100	0	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	75%	100	0	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	50%	100	3	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	25%	100	50	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	25%	100	52	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	50%	100	12	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Ethanol	25%	100	24	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	90%	100	9	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	75%	100	12	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	50%	100	81	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	1,4-Dioxane	25%	100	110	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	90%	100	7	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	75%	100	26	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	50%	100	44	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	75%	100	6	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	90%	100	11	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	75%	100	78	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	50%	100	125	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	25%	100	123	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	90%	100	9	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	75%	100	97	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	50%	100	112	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	25%	100	101	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	90%	100	5	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	127	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	91	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	112	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	90%	100	13	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	75%	100	51	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	50%	100	139	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Triglyme	25%	100	133	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	90%	100	107	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	75%	100	113	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	50%	100	129	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	75%	100	29	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	75%	100	11	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	28	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	121	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	90%	100	11	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	75%	100	58	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100	104	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Acetone	25%	100	103	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	90%	100	13	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Glycerol	25%	100	123	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100	42	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Isopropanol	25%	100	100	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	75%	100	12	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100	67	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Methanol	25%	100	128	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 4°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	90%	100	10	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	75%	100	12	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	34	%	Incubation: 0.4 M Tris-HCl, Assay: 0.1 M Tris-HCl	8	Incubation: 30°C, Assay: 37°C	0.8 mM L-Ala-Pro-p-nitroanilide hydrochloride	L-alanyl-L-proline, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase ("diluted in aqueous solution to test its activity")

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Visualization : Stability — Incubation

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Structure

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