Esterase (gene est4) from a marine mud metagenomic library
Enzyme Description
Sequence
MLVLWLLFLFFMSMLGLVLFASFSNKKAEQFLPPAGQFAKLSQGTLHYLDEGQGPAVVLVHGLAGNFHNFTYGVSKPLSAQYRVIAVDRPGCGYSKRNPHADASLEAQADTLIELLDHLKIESAVFVGHSLGGAISLAAAQRHPSRIKALALIAPLTHQPESPAPVFKPLDIPSPVLRKLIGWTFAVPGTLLRMSTSLKFIFGPEKAPADFAIRGGGILALKPQTFITASSDLQQVKACMPNIEAAYASMTTPVHVLYGREDRILSAKLNGEDLAQRLPGTQLTLINGGHMLPVTQAEVTCQFIAGVAGKATGLAP
Wenyuan Gao et al. (2016)
β A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters
Microbial Cell Factories
Β· doi:10.1186/s12934-016-0435-5 β
Β· Stability - Incubation
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Database ID
UniParc: UPI0006CE451D β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (39 measurements)
39 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | n-Hexane | 20% | 100.0 | 53.1 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 20% | 100.0 | 92.1 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 20% | 100.0 | 90.6 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 20% | 100.0 | 102.0 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 20% | 100.0 | 53.1 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 20% | 100.0 | 96.2 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 20% | 100.0 | 81.3 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Benzene | 20% | 100.0 | 37.9 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Toluene | 20% | 100.0 | 63.5 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Cyclohexane | 20% | 100.0 | 80.1 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 82.8 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | n-Heptane | 20% | 100.0 | 66.1 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isooctane | 20% | 100.0 | 85.5 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 61.7 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 50% | 100.0 | 19.4 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 50% | 100.0 | 23.2 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 50% | 100.0 | 0.5 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 50% | 100.0 | 0.0 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 50% | 100.0 | 7.8 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.