β-galactosidase from Aspergillus oryzae

Enzyme Description

Species

Extremophile

EC Number

3.2.1.12 ↗

Sequence

Length: 1005 amino acids

MKLLSVAAVALLAAQAAGASIKHRLNGFTILEHPDPAKRDLLQDIVTWDDKSLFINGERIMLFSGEVHPFRLPVPSLWLDIFHKIRALGFNCVSFYIDWALLEGKPGDYRAEGIFALEPFFDAAKEAGIYLIARPGSYINAEVSGGGFPGWLQRVNGTLRSSDEPFLKATDNYIANAAAAVAKAQITNGGPVILYQPENEYSGGCCGVKYPDADYMQYVMDQARKADIVVPFISNDASPSGHNAPGSGTGAVDIYGHDSYPLGFDCANPSVWPEGKLPDNFRTLHLEQSPSTPYSLLEFQAGAFDPWGGPGFEKCYALVNHEFSRVFYRNDLSFGVSTFNLYMTFGGTNWGNLGHPGGYTSYDYGSPITETRNVTREKYSDIKLLANFVKASPSYLTATPRNLTTGVYTDTSDLAVTPLIGDSPGSFFVVRHTDYSSQESTSYKLKLPTSAGNLTIPQLEGTLSLNGRDSKIHVVDYNVSGTNIIYSTAEVFTWKKFDGNKVLVLYGGPKEHHELAIASKSNVTIIEGSDSGIVSTRKGSSVIIGWDVSSTRRIVQVGDLRVFLLDRNSAYNYWVPELPTEGTSPGFSTSKTTASSIIVKAGYLLRGAHLDGADLHLTADFNATTPIEVIGAPTGAKNLFVNGEKASHTVDKNGIWSSEVKYAAPEIKLPGLKDLDWKYLDTLPEIKSSYDDSAWVSADLPKTKNTHRPLDTPTSLYSSDYGFHTGYLIYRGHFVANGKESEFFIRTQGGSAFGSSVWLNETYLGSWTGADYAMDGNSTYKLSQLESGKNYVITVVIDNLGLDENWTVGEETMKNPRGILSYKLSGQDASAITWKLTGNLGGEDYQDKVRGPLNEGGLYAERQGFHQPQPPSESWESGSPLEGLSKPGIGFYTAQFDLDLPKGWDVPLYFNFGNNTQAARAQLYVNGYQYGKFTGNVGPQTSFPVPEGILNYRGTNYVALSLWALESDGAKLGSFELSYTTPVLTGYGNVESPEQPKYEQRKGAY

S. Shubhada et al. (1993) — Borate ion-assisted stabilization of beta-galactosidase from Aspergillus oryzae by polyhydroxy compounds in water-miscible organic solvents

Enzyme and Microbial Technology · doi:10.1016/0141-0229(93)90102-8 ↗ · Stability - Incubation

144 measurements ▼

Database ID

UniProt: Q2UCU3 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (144 measurements)

144 measurements — page 3 of 8

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	100	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	99	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	8	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	102	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	10	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	30	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	29	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase

Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100	102	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	25%	100	103	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	60%	100	89	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100	99	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	25%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100	2	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100	90	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	25%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	60%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100	99	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	25%	100	99	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100	24	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	60%	100	4	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100	100	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	25%	100	90	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100	6	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	25%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	101	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	101	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	64	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	15	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	87	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	1	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	38	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	100	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	99	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	2	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	100	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	99	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	8	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	25%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	102	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	10	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	30	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	29	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	103	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	101	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	3	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	100	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	5	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	34	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	2	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	102	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	1	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	99	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	2	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	101	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	101	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	81	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	100	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	100	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	100	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100	3	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100	99	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	25%	100	95	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100	1	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	60%	100	2	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100	99	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	25%	100	2	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100	62	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	25%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	60%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100	100	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	25%	100	40	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	60%	100	0	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100	99	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	25%	100	5	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	60%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100	5	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	25%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	60%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10%	100	99	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25%	100	101	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100	75	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60%	100	21	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 4.6, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10%	100	99	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25%	100	100	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100	1	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60%	100	0	%	Incubation: sodium citrate-sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10%	100	57	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25%	100	2	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60%	100	1	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10%	100	100	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25%	100	100	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100	65	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60%	100	5	%	Incubation: sodium phosphate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 5.7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10%	100	89	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25%	100	69	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 7, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	10%	100	6	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	25%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100	0	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 25°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	60%	100	1	%	Incubation: Sodium borate buffer, Assay: 0.05 M sodium citrate-sodium phosphate and 1mM MgCl₂	Incubation: 8, Assay: 4.5	Incubation: 25°C, Assay: 25°C	2 mM o-Nitrophenyl β-D-galactoside	D-Galactose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase

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Visualization : Stability — Incubation

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