Esterase (gene Est1) from Psychrobacter pacificensis

Enzyme Description

Species

Psychrobacter pacificensis ↗

Extremophile

Yes "psychrophilic" organism

EC Number

3.1.1.1 ↗

Sequence

Length: 296 amino acids

MQNSYSHSLSNHKQLTQKSVNRCFNGEQHYYSHESTSTKTSMTFSIYLPDEALAGRRCPAILYLSGLTCNADNVTHKGHFQQKCSELGMILIAPDTSPRSSEDSDVPNDERYFVGQGASYYVDATQAPWSDNFNMQSYIVDELYDLLRSEFPIDSIGITGHSMGGHGALLLGFKFPSKFVSVSALSPVCAASESAWGQAAYTEYFGDDKSLWVQADAAQMIEKVGQQYASILVDQGAADNFLAEGQLQTSKLQDACAKAEQPLTLRYQAGHDHSYYFIQTVINDHIEHHWHMAQMS

Gaobing Wu et al. (2015) — A cold-adapted, solvent and salt tolerant esterase from marine bacterium Psychrobacter pacificensis

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.07.045 ↗ · Stability - Incubation

32 measurements ▼

Database ID

UniProt: A0A1G7A325 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	5%	100	60	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	30%	100	73	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	15%	100	29	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	10%	100	33	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	5%	100	43	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100	112	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	15%	100	165	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	156	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	5%	100	137	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	30%	100	133	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	15%	100	157	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	138	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number

Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Propanediol	5%	100	117	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Glycerol	30%	100	106	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Glycerol	15%	100	123	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Glycerol	10%	100	118	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Glycerol	5%	100	111	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100	46	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	15%	100	119	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100	119	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	5%	100	120	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	204	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	15%	100	194	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	161	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	5%	100	137	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Propanediol	30%	100	243	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Propanediol	15%	100	170	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Propanediol	10%	100	144	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	5%	100	117	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	30%	100	14	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	15%	100	38	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	10%	100	47	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	5%	100	60	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	30%	100	73	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	15%	100	29	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	10%	100	33	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	5%	100	43	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100	112	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	15%	100	165	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	156	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	5%	100	137	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	30%	100	133	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	15%	100	157	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number
Stability - Incubation	Activity after incubation (1 hour at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	138	%	? Incubation, 20 mM Tris-HCl	Incubation: ?, Assay: 7.5	Incubation: 4°C, Assay: 25°C	0.01 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Incorrect UniProt EC number

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Visualization : Stability — Incubation

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