Methionine adenosyltransferase from Pyrococcus furiosus

Enzyme Description

Species

Pyrococcus furiosus ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

2.5.1.6 ↗

Sequence

Length: 401 amino acids

MARNIVVEEIVRTPVEMQQVELVERKGIGHPDSIADGIAEAVSRALCREYIRRYGVILHHNTDQVEVVGGRAYPRFGGGEVVKPIYILLSGRAVELVDQELFPVHEVAIKAAKNYLKNAIRHLDVENHVIIDSRIGQGSVDLVSVFNKARENPIPLANDTSFGVGYAPLSETERLVLETEKLLNSEKFKKEYPAVGEDIKVMGLRRGNEIDLTIAAAIVDSEVATPKEYLEVKDKIKEAVEELAKEITSRKVNIYVNTADDPERGIYYITVTGTSAEAGDDGSVGRGNRVNGLITPNRHMSMEAAAGKNPVSHVGKIYNILAMLIAEDIAKTLPVEEVYVRILSQIGKPIDQPLVASIQVIPKPGHSVKEFEKDAYSIADEWLANITKVQKMILEDKISVF

Marina Porcelli et al. (2015) — A thermostable archaeal S -adenosylmethionine synthetase: a promising tool to improve the synthesis of adenosylmethionine analogs of biotechnological interest

Bioengineered · doi:10.1080/21655979.2015.1045170 ↗ · Activity - Classical Stability - Incubation

52 measurements ▼

Database ID

UniProt: Q8TZW1 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (52 measurements)

52 measurements — page 2 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	6	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	8	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	8	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	42	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	12	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	86	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	118	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	36	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	66	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	90	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	92	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	22	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	50	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	89	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase

Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	26	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Methanol	5%	100	89	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Methanol	10%	100	76	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Methanol	20%	100	65	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Methanol	30%	100	59	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Ethanol	5%	100	87	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Ethanol	10%	100	74	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Ethanol	20%	100	63	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Ethanol	30%	100	54	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Acetonitrile	10%	100	73	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Acetonitrile	20%	100	58	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Acetonitrile	30%	100	41	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	80	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	66	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	44	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC (amount of product L-methionine formation) in the presence of organic solvent	Acetonitrile	5%	100	84	%	10 mM Tris-HCl, 20 mM MgCl₂	8	50°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	13	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	64	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	16	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	6	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	8	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	8	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	42	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	12	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	86	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	118	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	36	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	66	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	90	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	92	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	22	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	50	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	89	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	13	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Isopropanol	50%	100	28	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	80	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 50°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 50°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	59	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	57	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	1-Propanol	50%	100	33	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 60°C), measured by HPLC	Isopropanol	50%	100	26	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Acetonitrile	50%	100	73	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (15 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	100	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Methanol	50%	100	28	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 60°C), measured by HPLC (amount of product L-methionine formation) in aqueous phase	Ethanol	50%	100	18	%	Incubation: 10 mM Tris-HCl, Assay: 10 mM Tris-HCl, 20 mM MgCl₂	Incubation: 8, Assay: 8	Incubation: 60°C, Assay: 80°C	ATP (Adenosine triphosphate), 5 mM L-methionine	S-adenosyl-L-methionine	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear assay in aqueous phase

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation