Alcohol dehydrogenase from Yokenella sp. WZY002

Enzyme Description

Species
Yokenella sp. WZY002 β†—
Extremophile
No
EC Number
1.1.1.2 β†—

Sequence

Length: 339 amino acids
MSIIKSYAAKEAGSELELYEYDAGELRPEDVEVQVDYCGICHSDLSMIDNEWGFSQYPLVAGHEVIGRVAALGSAAQEKGVKVGQRVGVGWTARSCGHCDACISGNQINCLEGAVATILNRGGFAEKLRADWQWVIPLPESIDIESAGPLLCGGITVFKPLLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPSKEQEVLAMGADKVVNSRDPDALNALAGQFDLIINTVNVDLDWQPYFEALAYGGHFHTVGAVMKPLPVPAFTLIAGDRSISGSATGTPYELRKLMKFAGRSKVSPTTELFPMSQINEAIQHVRDGKARYRVVLQADF
Xiangxian Ying et al. (2014) β€” Characterization of an Allylic/Benzyl Alcohol Dehydrogenase from Yokenella sp. Strain WZY002, an Organism Potentially Useful for the Synthesis of Ξ±,Ξ²-Unsaturated Alcohols from Allylic Aldehydes and Ketones
Applied and Environmental Microbiology  Β· doi:10.1128/AEM.03980-13 β†—  Β· Activity - Classical Stability - Incubation
54 measurements ▼
Database ID
UniProt: W6CX26 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 95 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 97 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (8 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 97 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 95 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (16 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 88 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 87 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 87 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20% 100 99 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20% 100 94 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20% 100 90 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (8 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20% 100 79 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20% 100 53 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (16 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20% 100 38 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Isopropanol 20% 100 27 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Ethanol 20% 100 90 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment, 340 nm) in aqueous phase Methanol 20% 100 100 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Methanol 20% 100 94 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Methanol 20% 100 99 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (8 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Methanol 20% 100 96 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurment) in aqueous phase Methanol 20% 100 93 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 20 mM 2-Buten-1-ol Crotonaldehyde 1 mM NADP+ β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
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Visualization : Activity β€” Classical

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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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