Lipase (gene lipB) from Serratia marcescens ECU1010

Enzyme Description

Species
Serratia marcescens ECU1010 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 614 amino acids
MGIFSYKDLDENASKALFSDALAISTYAYHNIGNGFDEGYHQTGFGLGLPLTLVTALIGSTQSQGGLPGLPWNPDSEQAAQEAVNNAGWSVISAAQLGYAGKTDARGTYYGETAGYTTAQAEVLGKYDSEGNLTAIGISFRGTSGPRESLIGDTIGDVINDLLAGFGPKGYADGYTLKAFGNLLGDAAKFAQAHGLSGEDVVVSGHSLGGLAVNSMAAQSDASWSGFYAQSNYVAFASPTQYETGGKVLNIGYENDPVFRALDGTTLTGTSLGVHDAPHTSATNNIVNFNDHYASDAWNLLPFSILNIPTWLSHLPFFYQDGLMRVLNSEFYSLTDKDSTIIVSNLSNITRGNTWVEDLNRNAETHSGPTFIIGSDGNDLIKGGKGNDYLEGRDGDDIFRDAGGYNLIAGGKGHNIFDTQQALKNTEVAYDGNTLYLRDAKGGITLADDISTLRSKETSWLIFNKEVDHQVTAAGLKSDSGLKAYAAAATGGDGDDVLQARSHDAWLFGNAGNDTLIGHAGGNLTFVGGSGDDVLKGVGNGNTFLFSGDFGRDQLYGFNASDKLVFIGTEGASGNIRDYATQQNDDLVLAFGHSQVTLIGVSLDHFSTDQVVLA
Su-Xia Li et al. (2014) β€” Essential Role of Gly33 in a Novel Organic Solvent-Tolerant Lipase from Serratia marcescens ECU1010 as Determined by Site-Directed Mutagenesis
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-013-0690-4 β†—  Β· Stability - Incubation
72 measurements ▼
Database ID
UniProt: D7SFB5 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (72 measurements)

72 measurements β€” page 2 of 4
Mutation Property Assay Solvent Solvent Volume Aqueous ReferenceWT Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 8.1 11.8 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100.0 57.6 59.6 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Diisopropyl Ether 99% 100.0 297.0 266.7 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Diisopropyl Ether 50% 100.0 316.8 347.3 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Diisopropyl Ether 10% 100.0 168.3 213.6 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 99% 100.0 33.7 39.9 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 50% 100.0 0.4 1.6 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 10% 100.0 30.7 35.0 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 99% 100.0 13.1 22.0 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 50% 100.0 0.7 5.8 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Diisopropyl Ether 99% 100.0 297.0 338.4 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
A187V Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10% 100.0 37.6 52.4 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 50% 100.0 1.8 2.7 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 99% 100.0 4.6 5.1 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 10% 100.0 51.5 47.2 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 50% 100.0 2.0 3.1 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 99% 100.0 50.7 58.3 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10% 100.0 37.6 34.1 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 50% 100.0 0.7 3.6 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
T270A Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 99% 100.0 13.1 17.7 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM potassium phosphate buffer Incubation: 8, Assay: 7.5 Incubation: 30Β°C, Assay: 30Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
β€Ή Prev
1 2 3 4
Next β€Ί

Mutations in this dataset (4)

WT G33D A187V T270A

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. β€” β€” β€” Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*