Cephalosporin C deacetylase from Bacillus subtilis CICC 20034

Enzyme Description

Species

Bacillus subtilis CICC 20034 ↗

Extremophile

EC Number

3.1.1.41 ↗

Sequence

Length: 318 amino acids

MQLYDLPLDQLQTYKPNKTAPHDFSDFWASSLHELAKEEAKPELKAESYPADGVKVFRLTYRSFGKAEIEGWYAVPDRQGPHPAIVKYHGYNASYDGDIHDIVNWALHGYAAFGMLVRGQHSSTDTSVSPHGHVPGWMTKGILDKDTYYYRGVYLDAVRALEVISGFDEVDETRIAVIGGSQGGGLSIAAAALSDIPRAVAADYPYLSNFERAIDVALDEPYLEINSFFRKNGSPETEKTAMNTLAYFDIMNLADRVKVPVLMSIGLIDRVTPPSTVFAAYNHLETEKQLKVYRYFGHEYIPSFHTEKLAFLKAHLKG

Qianqian Tian et al. (2013) — A novel cephalosporin deacetylating acetyl xylan esterase from Bacillus subtilis with high activity toward cephalosporin C and 7-aminocephalosporanic acid

Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5056-x ↗ · Stability - Incubation

21 measurements ▼

Database ID

UniProt: M1JUH6 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (21 measurements)

21 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase	Methanol	10%	100.0	165.0	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 40°C, Assay: 40°C	1.6 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Isopropanol

30%

100.0

154.0

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

n-Hexane

50%

100.0

116.94

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Toluene

50%

100.0

92.95

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Benzene

50%

100.0

98.43

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Isopropanol

50%

100.0

114.25

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Acetone

50%

100.0

1.64

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Methanol

50%

100.0

6.67

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

100.0

32.01

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

n-Hexane

30%

100.0

118.77

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Toluene

30%

100.0

104.5

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Benzene

30%

100.0

172.86

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10%

100.0

108.3

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Acetone

30%

100.0

93.85

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Methanol

30%

100.0

79.5

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Dimethyl Sulfoxide (DMSO)

30%

100.0

78.2

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

n-Hexane

10%

100.0

114.89

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Toluene

10%

100.0

139.1

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Benzene

10%

100.0

133.1

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Isopropanol

10%

100.0

124.58

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Acetone

10%

100.0

160.87

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

Stability - Incubation

Activity after incubation (24 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405) in aqueous phase

Methanol

10%

100.0

165.0

Incubation: ?, Assay: 50 mM potassium phosphate buffer

Incubation: ?, Assay: 7

Incubation: 40°C, Assay: 40°C

1.6 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 200 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about control

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Visualization : Stability — Incubation

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Structure

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Cephalosporin C deacetylase from Bacillus subtilis CICC 20034

Enzyme Description

Sequence

Experimental Data (21 measurements)

Visualization : Stability — Incubation

Structure

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