Esterase (gene RBest1) from a forest soil metagenomic library

Enzyme Description

Species

Na (forest soil metagenomic library)

Extremophile

EC Number

3.1.1.1 ↗

Sequence

Length: 286 amino acids

MSHLKTLSAFVVAAAAFGLGMPAQAAETLVEENVSYAKLDGTDLKLDIARPDGDGPYPAIVFIHGGGWYQGSRQRYRETIREAARRGYVGITITYRLMKFDEAKKETTKATPNFPAQIQDAKAALRWVRANSKKYHIDADHIGVTGESAGGHLSLPVGLKDAKAGLEGDAGNAEQSSRVQAVVNVFGPTDMEQCFKTSSVAWIFRLFMGGTPEEAAETYRVASPITYVSSDDPPVLTIHGDKDALVPIAQATMLDDKMKSAGAKHTLLLLKGQGHGFAGQAQQFCE

Renaud Berlemont et al. (2013) — Novel organic solvent-tolerant esterase isolated by metagenomics: insights into the lipase/esterase classification

Revista Argentina de Microbiología · PMID: 23560782 ↗ · Stability - Incubation

29 measurements ▼

Database ID

UniParc: UPI0001818360 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli RR1

Experimental Data (29 measurements)

29 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	Dimethylformamide (DMF)	25%	100.0	296.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100.0	535.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	n-Hexane	25%	9.0	23.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	n-Pentane	25%	9.0	35.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	p-Xylene	25%	9.0	37.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	Toluene	25%	9.0	33.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	Benzene	25%	9.0	100.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	Acetonitrile	25%	9.0	0.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%
Stability - Incubation	Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase	Dimethylformamide (DMF)	25%	9.0	7.0	%	Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1.25 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	Yes incubation with "Rotational agitation"	Water-incubated control (in %), assay in aqueous phase \| Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

n-Pentane

25%

100.0

501.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

p-Xylene

25%

100.0

167.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Benzene

25%

100.0

201.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Acetonitrile

25%

100.0

0.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethylformamide (DMF)

25%

100.0

20.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

100.0

116.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

n-Hexane

25%

0.59

0.8

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

n-Pentane

25%

0.59

1.2

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

p-Xylene

25%

0.59

1.7

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Toluene

25%

0.59

1.3

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Benzene

25%

0.59

2.1

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Acetonitrile

25%

0.59

0.2

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethylformamide (DMF)

25%

0.59

0.7

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

0.59

0.7

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

n-Hexane

25%

100.0

39.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

9.0

33.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

p-Xylene

25%

100.0

572.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Toluene

25%

100.0

535.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Benzene

25%

100.0

848.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Acetonitrile

25%

100.0

0.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethylformamide (DMF)

25%

100.0

296.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

100.0

535.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

n-Hexane

25%

9.0

23.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

n-Pentane

25%

9.0

35.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

p-Xylene

25%

9.0

37.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Toluene

25%

9.0

33.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Benzene

25%

9.0

100.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Acetonitrile

25%

9.0

0.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

Stability - Incubation

Activity after incubation (30 minutes at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment) in aqueous phase

Dimethylformamide (DMF)

25%

9.0

7.0

Incubation: 20 mM Tris-HCl, Assay: 20 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1.25 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Yes incubation with "Rotational agitation"

Water-incubated control (in %), assay in aqueous phase | Activity with p-Nitrophenyl butyrate as 100%

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Esterase (gene RBest1) from a forest soil metagenomic library

Enzyme Description

Sequence

Experimental Data (29 measurements)

Visualization : Stability — Incubation

Structure

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