Ξ±-amylase from Exiguobacterium sp. DAU5
Enzyme Description
Species
Extremophile
Yes
"mild halophilic" organism
EC Number
Sequence
MLKKRQGIAVLAGVTSIALLSGQPVAQAATPQNGTMMQYFEWYVPNDGLHWNHLSNDSQHLKDIGISTVWIPPAYKGTSQNDVGYGAYDLYDLGEFNQKGTTRTKYGTKAQLQSAISNLRGKGIGVYGDVVMNHKGGADYTESVQAVEVNPSNRNQETSGEYSISAWTGFNFAGRNNTYSPFKWRWYHFDGTDWDQSRSLSRIYKFKSTGKAWDSEVSGENGNYDYLMYADVDFEHPEVRQEMKNWGKWYADSLGLDGFRLDAVKHINHSYLKEWVTSVRQTTGKEMFTVAEYWKNDLGAINDYLAKTGYTHSVFDVPLHYNFQAAGNGGGYYDMRNILKGTVVEQHPTLAVTIVDNHDSQPGQSLESTVANWFKPLAYATIMTRGQGYPTLFYGDYYGTKGTTNREIPNMSGSLQPILKARKDFAYGTQHDYINHQDVIGWTREGVTDRAKSGLATILSDGPGGSKWMYVGKQNAGEVWKDMTGNNGRLVTINADGWGEFFVNGGSVSIYTQQ
Jie Chang et al. (2013)
β Recombinant Expression and Characterization of an Organic-Solvent-Tolerant Ξ±-Amylase from Exiguobacterium sp. DAU5
Applied Biochemistry and Biotechnology
Β· doi:10.1007/s12010-013-0101-x β
Β· Activity - Classical Stability - Half-life
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Database ID
UniProt: M4I6P6 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (32 measurements)
32 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40% | 100.0 | 44.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 88.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Chloroform | 40% | 100.0 | 16.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Chloroform | 20% | 100.0 | 67.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | 1-Butanol | 40% | 100.0 | 80.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | 1-Butanol | 20% | 100.0 | 85.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Acetone | 40% | 100.0 | 85.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Acetone | 20% | 100.0 | 94.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Ethanol | 40% | 100.0 | 60.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Ethanol | 20% | 100.0 | 97.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Acetonitrile | 40% | 100.0 | 75.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Acetonitrile | 20% | 100.0 | 82.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Methanol | 40% | 100.0 | 65.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Methanol | 20% | 100.0 | 94.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Dimethylformamide (DMF) | 40% | 100.0 | 31.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm), in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 81.0 | % | 50 mM glycine-NaOH buffer | 8.5 | 40Β°C | 1% w/v Starch | Reducing sugars | β | β | Classical aqueous control (in %) |
| Stability - Half-life | Half-life at 20Β°C, with remaining activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Chloroform | 40% | 32.0 | <1.0 | days | Incubation: 50 mM sodium phosphate, Assay: 50 mM glycine-NaOH buffer | Incubation: 7.4, Assay: 8.5 | Incubation: 20Β°C, Assay: 40Β°C | 1% w/v Starch | Reducing sugars | β | Incubation: 180 rpm | Classical aqueous control (in days) | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Half-life | Half-life at 20Β°C, with remaining activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 40% | 32.0 | <1.0 | days | Incubation: 50 mM sodium phosphate, Assay: 50 mM glycine-NaOH buffer | Incubation: 7.4, Assay: 8.5 | Incubation: 20Β°C, Assay: 40Β°C | 1% w/v Starch | Reducing sugars | β | Incubation: 180 rpm | Classical aqueous control (in days) | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Half-life | Half-life at 20Β°C, with remaining activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 40% | 32.0 | 4.0 | days | Incubation: 50 mM sodium phosphate, Assay: 50 mM glycine-NaOH buffer | Incubation: 7.4, Assay: 8.5 | Incubation: 20Β°C, Assay: 40Β°C | 1% w/v Starch | Reducing sugars | β | Incubation: 180 rpm | Classical aqueous control (in days) | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Half-life | Half-life at 20Β°C, with remaining activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 40% | 32.0 | 5.0 | days | Incubation: 50 mM sodium phosphate, Assay: 50 mM glycine-NaOH buffer | Incubation: 7.4, Assay: 8.5 | Incubation: 20Β°C, Assay: 40Β°C | 1% w/v Starch | Reducing sugars | β | Incubation: 180 rpm | Classical aqueous control (in days) | Uncertainty about whether activity was measured with or without organic solvent |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Half-life
One bar per measurement. Colour = solvent, shade = solvent volume.