Azoreductase from Shewanella oneidensis MR-1

Enzyme Description

Species
Shewanella oneidensis MR-1 β†—
Extremophile
No but "organic-solvent tolerant" enzyme
EC Number
1.7.1.17 β†—

Sequence

Length: 198 amino acids
MSKVLVLKSSILGGYSQSALLVDYLIGKWEKQGATITVRDLAGKDVLPMVDGEIASGLRGGAELTARQQEMLDLSNALVEELKANDTIVITAPMYNFNIPTQLKNWIDFVARAGVTFTYTENGPKGLVEGKRAVLITTRGGAHKDGPTDHMVPFLKTFLGFIGITDVDVVYAEALNMGPEANQKGISEAKASIDKLAV
Yuyi Yang et al. (2012) β€” Characterization of an efficient catalytic and organic solvent-tolerant azoreductase toward methyl red from Shewanella oneidensis MR-1
Environmental Science and Pollution Research  Β· doi:10.1007/s11356-012-1221-5 β†—  Β· Stability - Incubation
48 measurements ▼
Database ID
UniProt: Q8E990 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 70% 100.0 41.36 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Acetone 50% 100.0 21.23 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Methanol 50% 100.0 4.25 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Ethanol 50% 100.0 1.39 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Isopropanol 50% 100.0 1.06 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase 1-Propanol 50% 100.0 0.0 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 60% 100.0 82.8 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Acetone 60% 100.0 3.6 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Methanol 60% 100.0 3.07 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Ethanol 60% 100.0 0.0 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Isopropanol 60% 100.0 0.0 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase 1-Propanol 60% 100.0 0.0 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 82.29 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Acetone 70% 100.0 3.43 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Methanol 70% 100.0 2.31 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Ethanol 70% 100.0 0.0 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Isopropanol 70% 100.0 0.0 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase 1-Propanol 70% 100.0 0.0 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 80% 100.0 4.47 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (decrease in methyl red absorbance measurement) in aqueous phase Acetone 80% 100.0 1.83 % Incubation: ?, Assay: 50 mM citrate-phosphate Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: Room temperature 0.15 mM Methyl red Reduced amine products from Methyl red 0.05 mM NAD(P)H β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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