Cytochrome P450 2C9 from Homo sapiens

Enzyme Description

Species

Extremophile

EC Number

1.14.14.1 ↗

Sequence

Length: 490 amino acids

MDSLVVLVLCLSCLLLLSLWRQSSGRGKLPPGPTPLPVIGNILQIGIKDISKSLTNLSKVYGPVFTLYFGLKPIVVLHGYEAVKEALIDLGEEFSGRGIFPLAERANRGFGIVFSNGKKWKEIRRFSLMTLRNFGMGKRSIEDRVQEEARCLVEELRKTKASPCDPTFILGCAPCNVICSIIFHKRFDYKDQQFLNLMEKLNENIKILSSPWIQICNNFSPIIDYFPGTHNKLLKNVAFMKSYILEKVKEHQESMDMNNPQDFIDCFLMKMEKEKHNQPSEFTIESLENTAVDLFGAGTETTSTTLRYALLLLLKHPEVTAKVQEEIERVIGRNRSPCMQDRSHMPYTDAVVHEVQRYIDLLPTSLPHAVTCDIKFRNYLIPKGTTILISLTSVLHDNKEFPNPEMFDPHHFLDEGGNFKKSKYFMPFSAGKRICVGEALAGMELFLFLTSILQNFNLKSLVDPKNLDTTPVVNGFASVPPFYQLCFIPV

Vanessa González-Pérez et al. (2012) — Impact of Organic Solvents on Cytochrome P450 Probe Reactions: Filling the Gap with (S)-Warfarin and Midazolam Hydroxylation

Drug Metabolism and Disposition · doi:10.1124/dmd.112.047134 ↗ · Activity - Classical

54 measurements ▼

Database ID

UniProt: P11712 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host insect cells

Experimental Data (54 measurements)

54 measurements — page 2 of 3

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	100	23	18	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	100	23	22	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	0.1%	100	69	70	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	1%	100	14	4	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	0.1%	100	69	94	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	1%	100	14	10	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	1%	100	42	43	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	1%	100	42	31	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	2%	100	22	27	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	0.1%	—	47	64	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	2%	100	22	10	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	0.1%	100	47	50	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	2%	100	18	20	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	0.1%	100	76	84	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	0.1%	100	76	99	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	1%	100	66	62	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	1%	100	66	61	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	2%	100	62	44	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	2%	100	62	42	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	0.1%	100	79	88	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)

WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	0.1%	100	—	76	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	100	—	23	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	—	41	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.1%	100	—	62	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	0.1%	100	—	69	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	2%	100	—	5	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	1%	100	—	42	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	1%	100	—	20	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	0.1%	100	—	59	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	2%	100	—	22	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	2%	100	—	18	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	0.1%	100	—	47	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	1%	100	—	49	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	0.1%	100	—	79	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	1%	100	—	14	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	1%	100	—	66	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	2%	100	—	2	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	2%	100	—	62	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	2%	100	2	4	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	2%	100	2	2	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	100	23	18	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2%	100	23	22	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	0.1%	100	69	70	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	1%	100	14	4	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	0.1%	100	69	94	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	1%	100	14	10	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	1%	100	42	43	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	1%	100	42	31	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	2%	100	22	27	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	0.1%	—	47	64	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetone	2%	100	22	10	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Isopropanol	0.1%	100	47	50	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	2%	100	18	20	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	0.1%	100	76	84	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	0.1%	100	76	99	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	1%	100	66	62	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	1%	100	66	61	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	2%	100	62	44	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Acetonitrile	2%	100	62	42	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	0.1%	100	79	88	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	0.1%	100	79	91	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	1%	100	49	47	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	1%	100	49	20	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	41	40	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Methanol	2%	100	18	4	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	0.1%	100	59	54	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	0.1%	100	59	57	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	1%	100	20	15	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	1%	100	20	3	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	2%	100	5	2	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Ethanol	2%	100	5	3	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.1%	100	62	49	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
I359L	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.1%	100	62	78	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)
R144C	Activity - Classical	Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	41	34	%	100 mM potassium phosphate buffer	7.4	37°C	4 µM (S)-Warfarin	(S)-7-Hydroxywarfarin	1 mM NADPH	—	Classical aqueous control (in %)

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Mutations in this dataset (3)

WT R144C I359L

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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