Lipase from Geobacillus zalihae T1

Enzyme Description

Species
Geobacillus zalihae T1 β†—
Extremophile
Yes thermophilic organism
EC Number
3.1.1.3 β†—

Sequence

Length: 388 amino acids
ASLRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRIHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLEAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSVSGAEKLNQWVQASPNTYYLSFSTERTYRGALTGNHYPELGMNAFSAVVCAPFLGSYRNPTLGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTYNVDHLEIIGVDPNPSFDIRAFYLRLAEQLASLQP
Mohd. Shukuri Mohamad Ali et al. (2012) β€” Purification and Characterisation of an F16L Mutant of a Thermostable Lipase
The Protein Journal  Β· doi:10.1007/s10930-012-9395-8 β†—  Β· Stability - Incubation
13 measurements ▼
Database ID
UniProt: Q842J9 β†—
Sequence Annotation
Inferred - from protein name (27 first residues from UniProt sequence removed from mature protein, 1 mutation)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (13 measurements)

13 measurements
Mutation Property Assay Solvent Solvent Volume Aqueous ReferenceWT Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) Methanol 30% 100 β€” 90 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) Ethanol 30% 100 β€” 56 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) Acetone 30% 100 β€” 83 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) 1-Propanol 30% 100 β€” 53 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) 1-Butanol 30% 100 β€” 88 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) Propyl Acetate 30% 100 β€” 74 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) Benzene 30% 100 β€” 76 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) 1-Heptanol 30% 100 β€” 77 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) 1-Octanol 30% 100 β€” 60 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) n-Hexane 30% 100 β€” 85 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) n-Heptane 30% 100 β€” 81 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (Kwon-Rhee colorimetric assay , free fatty acids and copper II acetate reaction product absorbance measurement, 715 nm) Isooctane 30% 100 β€” 82 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent
F16L Stability - Incubation Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry n-Hexadecane 30% 100 β€” 70 % Incubation: 50 mM Tris-HCl, Assay: 50 mM phosphate buffer, 0.01 mM CaCl2 Incubation: 8, Assay: 7 Incubation: 50Β°C, Assay: 70Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay done with or without organic solvent

Mutations in this dataset (1) β€” Mohd. Shukuri Mohamad Ali et al. (2012)

F16L

Visualization : Stability β€” Incubation

Mohd. Shukuri Mohamad Ali et al. (2012)

One bar per measurement. Colour = solvent, shade = solvent volume.

Roswanira Abdul Wahab et al. (2012) β€” Manipulation of the Conformation and Enzymatic Properties of T1 Lipase by Site-Directed Mutagenesis of the Protein Core
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-012-9728-2 β†—  Β· Activity + Stability - Incubation
56 measurements ▼
Jonathan Maiangwa et al. (2017) β€” Lid opening and conformational stability of T1 Lipase is mediated by increasing chain length polar solvents
PeerJ  Β· doi:10.7717/peerj.3341 β†—  Β· Activity + Stability - Incubation
10 measurements ▼
Siti Nor Hasmah Ishak et al. (2019) β€” Changes of Thermostability, Organic Solvent, and pH Stability in Geobacillus zalihae HT1 and Its Mutant by Calcium Ion
International Journal of Molecular Sciences  Β· doi:10.3390/ijms20102561 β†—  Β· Stability - Incubation
44 measurements ▼
Samah Hashim Albayati et al. (2025) β€” Insight into improved specificity and thermostability of Geobacillus zalihae T1 lipase by introducing novel molecular interactions via phenylalanin to cysteine substitution
3 Biotech  Β· doi:10.1007/s13205-025-04330-5 β†—  Β· Stability - Incubation
18 measurements ▼

Structure

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