Esterase (gene EstC23) from a soil metagenomic library

Enzyme Description

Species
Na (soil metagenomic library)
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 296 amino acids
MSQQQLESIIQMLKSQPIAGKPSIAETRAGFEQMAAMFPVEADVKSEPVNAGGVKSEWVTAPGADPGRAVLYLHGGGYVIGSISTHRSFAGRISRAAKARVLVIDYRLAPEHPFPAAVEDSVAAYRWMLSTGLKPSRIAVAGDSAGGGLTVATLVAIRDAKLPVPAAGVALSPWVDMEGVGDSMKTKAAVDPMVQKDGLIEMAKAYLGGKDPRTPLAAPLYADLAGLPPLLIQVGTAETLLDDSTRLAERARKAGVKVTLEPWENMVHVFQIFASILDEGQQAIDKIGAFIRANAE
Peng Jin et al. (2012) β€” Overexpression and characterization of a new organic solvent-tolerant esterase derived from soil metagenomic DNA
Bioresource Technology  Β· doi:10.1016/j.biortech.2011.10.087 β†—  Β· Stability - Incubation
54 measurements ▼
Database ID
UniProt: H8ZRT5 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements β€” page 3 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Toluene 50% 100.0 259.9 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 20% 100.0 213.57 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase p-Xylene 50% 100.0 249.86 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 20% 100.0 192.71 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 50% 100.0 285.06 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 20% 100.0 133.13 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 20% 100.0 74.61 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 20% 100.0 88.1 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 20% 100.0 150.95 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (48 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100.0 169.87 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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