Lipase LipC12 from a wastewater treatment plant lagoon metagenomic library

Enzyme Description

Species

Na (wastewater treatment plant lagoon metagenomic library)

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 293 amino acids

MSASSLKFPIVLVHGLLGFDKIGGIYPYFYGIKEALEKAGAKVYIATLSALNSNELRGEQLLEFVRKVQAETGAAKVNLIGHSQGPLACRYVAATHPELIASVTSVNGVNHGSEVADLVRLALTPGRLPESIANAAMSAFGQLLSALAGSPRLPQSGIEALEALTSEGVAAFNNKYPQGLPAEWGGEGKELVNGVYYYSWSGVIDYNPLHQGANNLDPLHVAMLAFSILFTNERFQNDGLVGRYSSHLGKVIGSDYSMDHVDAINQLAGVVANNTDPVQLFVEHVARLKSKGL

Arnaldo Glogauer et al. (2011) — Identification and characterization of a new true lipase isolated through metagenomic approach

Microbial Cell Factories · doi:10.1186/1475-2859-10-54 ↗ · Stability - Incubation

22 measurements ▼

Database ID

UniParc: UPI00021B5F20 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	15%	100.0	587.6	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	30%	100.0	1561.1	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay

Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Tetrahydrofuran (THF)	30%	100.0	1690.6	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	30%	100.0	316.2	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	15%	100.0	62.7	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100.0	355.9	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethylformamide (DMF)	15%	100.0	223.1	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	770.1	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	15%	100.0	263.7	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1,4-Dioxane	30%	100.0	509.7	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1,4-Dioxane	15%	100.0	505.4	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	30%	100.0	624.2	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	15%	100.0	66.5	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	15%	100.0	1204.1	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Tetrahydrofuran (THF)	15%	100.0	463.6	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Glycerol	30%	100.0	233.0	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Glycerol	15%	100.0	167.8	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	30%	100.0	696.2	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	15%	100.0	170.6	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Propanol	30%	100.0	1161.9	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Propanol	15%	100.0	329.0	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	30%	100.0	428.4	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	15%	100.0	587.6	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay
Stability - Incubation	Activity after incubation (48 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	30%	100.0	1561.1	%	Incubation: 200 mM Tris-HCl, 5 mM CaCl2, Assay: 50 mM Tris-HCl, 1 mM CaCl2, 0.3% (v/v) Triton X-100, 1% acetonitrile, 4% isopropanol	Incubation: 8, Assay: 7.5	Incubation: 4°C, Assay: 30°C	1 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Highly diluted organic solvent so aqueous phase for assay

‹ Prev

1 2

Next ›

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*